| Objective:To investigate①the clinical effect of super-micro Black Dihuang decoction on Chronic Kidney Disease (CKD) 3 degree.②the influence of super-micro Black Dihuang decoction on behavior, posture, weight and pathological kidney of UUO rats.③the effect of super-micro Black Dihuang decoction on apoptosis and renal organization Bcl-2,Bax of Unilateral Ureteral Obstruction (UUO) rats.④the effect of super-micro Black Dihuang decoction on serum FN,ColⅣ,TGFβ1 of Unilateral Ureteral Obstruction (UUO) rats.Methods:Clinical study:60 CKD 3 degree patients were divided randomly into the Super-micro group and Niaoduqing group,compared the 24-hour urinary protein quantitative,serum creatinine, urea nitrogen and the efficacy before and after treatment. Animal experiment:40 Unilateral Ureteral Obstruction (UUO) SD rats were randomized into Control group, Sham operation group, Model group, Super-micro group and Niaoduqing group. After 14 days, next indicators of each group rats were detected:①behavior, posture, weight and pathological kidney of UUO rats.②the concentration of Bcl-2 and Bax with immunohistochemistry method, and compared with the apoptosis of renal organization with TUNEL method③the rat kidney morphology of each group.④the serum creatinine, urea nitrogen and 24-hour urine protein of each group.⑤the concentration of FN,ColⅣin serum with enzyme-linked timmunosorbent assay (ELISA) method, and the concentration of TGFβ1 with immunohistochemistry method.Results:The total effective of Super-micro group and the Niaoduqing group is similar (P>0.05). Both two groups were improved On serum creatinine, urea nitrogen and 24-hour urine protein quantitative (P<0.05), but no difference on hemoglobin and no significant difference between Super-micro group and the Niaoduqing group in the improvement of serum creatinine (P>0.05), urea nitrogen to improve in Niaoduqing group is better than that of Super-micro group (P<0.05), while the 24-hour urinary protein of Super-micro group to improve was better than Niaoduqing group (P<0.05). The behavior, posture and weight changes of model group were significantly different from the other group, In pathology, Super-micro group was simila with Niaoduqing group, but significantly different from the model group. Super-micro Black Dihuang decoction on renal function and urinary protein were clearly improved effect (P<0.05). Experimental study also was found a similar effect (P> 0.05) between Super-micro rats group and Niaoduqing rats group, while serum FN,ColⅣconcentrations of Super-micro Black Dihuang decoction were found similar levels as those of Niaoduqing (P> 0.05), but much lower than that of the model group (P<0.05). Bcl-2 of Super-micro group and Niaoduqing group were higher than model group (P<0.05), while Bax and TGFβ1 of Super-micro group and Niaoduqing group were lower than the model group (P<0.05). The apoptosis of Super-micro group and Niaoduqing group are lower than that of the model group (P <0.05).Conclusion:①Super-micro Black Dihuang decoction group with Spleen and Kidney deficiency type can be treated safely and effectively, whose effect is similar with that of Niaoduqing.②The behavior, body weight and renal pathology of Super-micro Black Dihuang decoction group has a clear role.③Super-micro Black Dihuang decoction can reduce the 24-hour urine protein, improve kidney function.④Supermicro Black Dihuang decoction can inhibit FN, ColⅣ, TGFβ1, which can reduce the apoptosis of renal.
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