| Breast cancer is a kind of disease threatening women. The increasing number of the breast cancer patients and the high mortality rate have been attracting the attentions of the World Health Organization and doctors. And in China, the patients of the breast cancer are characterized by the younger women that aged forty to fifty, while those are in their best time of working. And this festher exerts high influence on the patient's family and society. Thus it is a hot pot to look for the effective medicine to treat breast cancer.The modern medicine studies show that the mutant of p53 gene is involved in breast cancer, which is related to the p21 gene. The p21 gene can regulate the expression of cyclin D1 and cyclin E. While the abnormal expression of p21 gene, cyclin D1, and cyclin E are the frequent events in the early time of breast cancer, which suggest the loss control of cell cycle is an important factor in the happening and developing of the breast cancer.Traditional Chinese medicine (TCM) is an important mine and it has had a 2,500 year's history for TCM to adopt 100,000 different formulae to prevent and treat various diseases. The breast cancer was described and treated long time ago. Even though TCM has its own advantages of treating breast cancer and has been demonstrating good effectiveness in clinical practice, the dim effective constituents of many TCM-based formulae and their unclear action mechanisms have been restricting themselves in spreading. Therefore, it is necessary and possible, with modern technology and combining the multi-suject methods, to elucidate the effective constituents and mechanisms of those TCM-based formulae and thereafter spreading their applications. XiaoYanYe (XYY) is a formula based on traditional Chinese meicine theories and it has been showing good clinical efficacy that can increase the five years survival rate of breast cancer patients in clinical trials. For elucidating the mechanisms of XYY and spreading its applications, modern biotechnology, proteomics and pharmcological technologies have been adopted to explore the mechanisms and molecular targets of XYY.To observe the effects of traditional Chinese medicine XYY on breast cancer beared mice, Balb/C female mice were planted with breast cancer cells 4TO7. After 50 days administration of XXY, the size of the tumor was measured. And results showed not only the size and weight were reduced, but also the increased spleen and lung index were inhibited, which suggest that XXY can inhibit the growth of breast cancer in mice. In in vitro study, serum pharmacologic techniques were adopted, and the proliferation inhibitive effects of XYY on breast cancer cell were observed with SRB, MTT, flow cytometry and westernblot. The results showed that XYY can evidently inhibit both breast cancer cell lines proliferation. The further studies displayed that XYY can influence on the cell cycle. The detailed mechanisms of XYY inhibiting breast cancer cell line proliferation are attributed to the influence of XYY on the cell cycle regulators and microtubes. And the enzyme spectrum analysis on the cell showed that XYY can also control the activation of the VEGFR, which is further confirmed by the cell migration and the cell invasion experiments. All these results suggested XYY is able to inhibit breast cancer..It is first time that the antitumor mechanisms of XYY were elucidated from in vitro, cellular and molecular level through compositive technology systems. This study opens up a good source for the breast cancer treatment and possesses important theoretical value.
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