Research On The Effect And Mechanism Of Electro-acupuncture On Characteristics Of Temporal Dynamics Of The Mood In Model Of Chronic Jet Lag

Objective:The aim of this study was to investigate the Effect and Mechanism of Electro-acupuncture (EA) on Characteristics of Temporal Dynamics of the Mood in Syrian hamster Model of Chronic Jet Lag.Methods:Model of chronic jet lag was established by appling the methods of advances of light/dark cycle for 10 days. EA (15/25Hz in frequency, 1-3v) was applied to "Baihui" (Du20) and "Changqiang" (Dul) for 15min/d and lasted for 3 days. The behavioral tests were measured by the elevated plus-maze test (EPM) and open field test (OF) in ZTO(8:00),ZT4(12:00),ZT8(16:00),ZT12 (20:00),ZT16 (24:00),ZT20 (4:00).The expressions of AVPmRNA and CRHmRNA were measured by PR-PCR method. The level of AVP in the pituitary gland was measured by in radio-immunity. The content of CORT was detected by ELISA method.Results:(1) Compared with the control group, the levels of anxiety and depression were increased in model group. Double-peak and double-valley characteristics of temporal dynamics of the mood (similar ultradian rhythms) could be observed in control group.Abnormal characteristics of temporal dynamics of the mood were represented in the model group (eg.phase shift,antiphase of the peak performance phase, the midvalues decrease, and the amplitude large or small). (2) Abnormal characteristics of temporal dynamics of the mood was represented in the Natural recovery group (eg.Circadian rhythm with single peak). (3) Double-peak and double-valley characteristics of temporal dynamics of the mood (similar ultradian rhythms) was appeared in EA group.(4) Compared with the model group and the Natural recovery group, AVPmRNA in the SCN and AVP in the pituitary were were obviously increased, AVPmRNA in the hypothalamus and CORT in serum were displayed a recovery trend by EA.Conclusion:Double-peak and double-valley characteristics of temporal dynamics of the mood was showed in control group. Abnormal characteristics of temporal dynamics of the mood was displayed in the model group and the natural recovery group(eg.disappearance and phase shift of Double-peak and double-valley, or single peak). Double-peak and double-valley temporal dynamic structure of the mood can be restored by EA. The mechanism of EA may be correlated with affection AVP level and gene expression in the center, regulating HPA axis function.