| Objective:To investigate the effect of the modified decoction of elevating the spleen yang reinforcing stomach which induced from the method of supplementing spleen and kidney activating the blood circulation raise yang and release dampness(SAR) onα-smooth muscle actin(α-SMA),Vascular endothelial growth factor( VEGF),Connective tissue growth factor( CTGF),CTGFmRNA of kidney tissue of adriamycin-induced nephrotic rats, and to illustrate the mechanism of the modified decoction of elevating the spleen yang reinforcing stomach on protecting renal tubular interstial lesion.Methods:In the experiment,the rats by tail vein injection of adriamycin were adopted.The rats were randomly divided into the normal control group (n=10),the model control group (n=10),the low dosage chinese medicine group(n=15),the high dosage chinese medicine group (n=15)and the lotensin group(n=15).It is observed for the change of the common physical order,24h urinary Protein level,blood urea nitrogen(Bun) and serum creatinine(Scr) of experimental rats.HE and Masson staining method for pathologic slice are used to observe the pathologic change of the experimental rats, kidney tissue after treated and the phase of renal fibroblast.The expression ofα-SMA,VEGF,CTGF of renal tissue were determined by immunohistochemistry methods.The expression of CTGFmRNA of renal tissue was determined by in situ hybridization.Results:1.Compared with the model control group,24h urinary Protein levels in the Chinese medicine group and lotensin group were markedly intervented at the end of ten weeks(P<0.05).2.Compared with the model control group,the level of blood urea nitrogen and serum creatinine in the Chinese medicine group and lotensin group were obviously decreased (P<0.05).3.Compared with other groups, the renal tubulointerstitial lesion degree in high dosage Chinese medicine group was ameliorated significantly (P<0.05).4.The expression ofα-SMA,CTGF in kidney tissue were decreased and the expression of VEGF was increased in the Chinese medicine group and the lotensin group which were significantly different with the control and model group (P<0.05).4.The expression of CTGFmRNA was also reduced which was significantly different with the control and model group (P<0.05).5.The renal tubulointerstitial lesion degree and the level of blood urea nitrogen and serum creatinine were closely related withα-SMA,VEGF and CTGF.Conclusions:1.Modified decoction of elevating the spleen yang reinforcing stomach could effectively reduced the 24h urinary protein of adriamycin-induced nephrotic rats.2.Modified decoction of elevating the spleen yang reinforcing stomach could inhibit the transdifferentiation of renal tubular epithelial and reduce the impairment of tubular interstial.3.Modified decoction of elevating the spleen yang reinforcing stomach can alleviate the renal interstitial lesion in adriamycin-induced nephrotic rats through decreasingα-SMA,CTGF and increasing the VEGF expression to reduce the impairment of tubulointerstitium.4.Modified decoction of elevating the spleen yang reinforcing stomach plays a remarkable role in preventing the development of tubular interstial lesion of adriamycin-induced nephrotic rats. |
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