Avp (4-8) A New Way Of Regulating Gene And Cct¦Â Rats In The Insect Cell Expression

Part1. The New Pathways by Which AVP4-8 Regulate GenesXu Kanyan ( Biochemistry )Directed by Prof. Du YucangThe neuropeptide AVP4-8 is a metabolite of argipressin which has been shown to have potent memory-enhancing activity. It can evoke a series of biophysical and biochemical events in rat brain. Using RT-PCR plus Southern Blot, we found that AVP4-8 could upregulate the mRNA level of CTP: phosphocholine cytidylyltransferase in rat hippocampal neurons and the mRNA level of c-fos in rat hippocampal astrocytes. It's antagonist, ZDC(C)PR could inhibit the process. Using Actinomycin-D to inhibit the cell's transcription, we found AVP4-8 could prolong the halflife of CCT mRNA and c-fos mRNA, which suggesting the role of posttranscriptional regulation in AVP4-8's effect on some genes' mRNA levels.We also found the upregulation of CCT mRNA led to the increase of enzymatic activity, this further proved the speculation that CCT may play animportant role in some of the AVP4-8 's functions.