Analysis Of Fatty Acid Esters Of Hydroxy Fatty Acids In Life Cycle

FAHFAs(fatty acid esters of hydroxy fatty acids)are a class of important endogenous lipids,which show great promise for treating diabetes and inflammatory diseases.Since its discovery in 2014,the research of FAHFAs has attracted the attention worldwide.In recent years,scientists have made some research about FAHFAs on the physiological functions,metabolic pathways,storage forms in the tissue and cells,development of analytical methods and discovery of new FAHFAs,and acquired some preliminary results.However,the understanding of FAHFAs is still superficial.Exploration on the more unknown physiological functions of FAHFAs is of great significance to provide insight into FAHFAs.Lipids are important component of life and possess many crucial physiological functions including signaling,forming membrane matrices,and storing energy.It is essential for the growth and development of animals and plants.The lipids alternations are closely related to the growth and aging process of life.In mammals,lipids participate in growth and aging by regulating cell growth and senescence.In plants,lipids also participate in the whole life process of plant germination,growth,differentiation,and maturation.As a new class of lipids,the relationship between FAHFAs and the growth and aging of life has not been reported.Based on the above analysis,by using stable isotope labeling-assisted liquid chromatography-mass spectrometry as major research method,mice 、rice and arabidopsis thaliana as research object,we explored the relationship between FAHFAs and the life cycle on the molecular level.The specific contents are as follows:1.Based on developed FAHFAs screening approach,we investigated FAHFA families and regioisomers species and content variation in the visceral adipose tissue(VAT)from mice of different ages and found that FAHFA families and regioisomers numbers increased with age.In addition,there were obvious differences in contents of FAHFAs in young,middle-aged,and old mice VAT.In middle-aged mice,the relative concentration of FAHFAs in 100 mg of VAT was lower than that in young and/or old mice,while the total amount of FAHFA was higher in middle-aged mice.This is the first work to suggest that FAHFAs have a close relationship with life development and aging process,providing important clues for the research and exploration of FAHFAs physiological function.2.Based on SAX-SPE-SIL-UHPLC-MS/MS analysis strategy,we established quantitative methods for 20 FAHFAs regioisomers in different tissues of rice and arabidopsis thaliana.Using this method,14 FAHFAs regioisomers were successfully detected in rice and arabidopsis thaliana of different growing stage,and the spatiotemporal distribution of the 14 FAHFA regioisomers were analyzed.This study for the first time obtained spatiotemporal distribution of FAHFAs in plants,and provided data support for further study of the working mechanism of FAHFAs in plants.3.Based on the established quantitative methods for 20 FAHFAs regioisomers in different tissues of rice,we explored the spatiotemporal distribution of FAHFAs in rice grown under Cd stress,and compared it with rice grown under normal environment,the results showed that the levels of FAHFAs were higher in rice grown under Cd stress.This work explored the influence of Cd stress on FAHFAs content in rice,providing a new clue for studying the relationship between heavy metal stress and FAHFAs in plants.