Structural Diversity Research Of Phvcobilisomes And Analysis On Early Development Of Green Tide In Yehow Sea

Phycobilisomes are cyanobacteria and red algae's the main light-harvesting protein-pigment complexes. It exists with bacterial chlorophyll, chlorophyll-protein-pigmentcomplex at the same time, the oxygen-evolving photosynthetic organisms, twolight-harvesting protein-pigment complex. Phycobilisomes and chlorophyll-proteinare different:1phycobilisomes are connected with reaction center by peptide linkerproteins,2the absorption of phycobilisome's light absorption range is450-650nm,and chlorophyll and bacteriochlorophyll have different spectra since red algae can livein a nearly200-meter-deep ocean, so it can be very efficient to capture low-light450-550nm light,3photosynthetic pigments (phycobilin) connect with proteincovalently while chlorophyll molecules connect with protein backbone throughintermolecular hydrogen bonds, van der Waals and other weak interactions.For phycobilisomes structure, the research began in the1970s. The technology ofelectron microscopy was applied, but limited due to technical conditions, theresolution is very low. As for the development of electron microscopy of biologicalsamples and cryo-electron microscopy techniques, it is possible to establish andmature, high-resolution structural analysis of biological macromolecules. Therefore,by transmission electron microscopy, existing known all types of phycobilisomesstructure was observed. Higher-quality of semi-ellipsoidal, semi-disc, bundle andblock phycobilisome were obtained. Moreover, we conducted a more in-depth studyof the thermophilic cyanobacterium Thermosynechococcus vulcanus and red algaPorphyridium aerugineum phycobilisomes. Thermosynechococcus vulcanusresurgence of two-dimensional average single particle analysis, we found that on itstriple-core shoulders, there are two proteins, presumably as a protein containingpigment on each shoulder. Frozen electron microscopy (cryo-EM) observationsfound that phycobilisomes particles mostly were dislocation arrangement in the gas- liquid interface, this polarity-the arrangement of non-polar interface may be similarto the intracellular membranes-outer membrane arrangement. Marine red algaPorphyridium aerugineum's two-dimensional average was obtained, making a veryimportant structure to reveal its three-dimensional structure by cryo-EM in-depthinformation and technical basis.Green tide in the Yellow Sea as part of a national emergency scientific andtechnological multi-level genetic research suggests may be the same population. Weproposed a temporary existence in the spring, the Yellow Sea stable habitat, providenutrition for Ulva prolifera, the optimum temperature for rapid growth andreproduction hypothesis. After the MODIS SSH and SST data analysis, we found thatthe existence of a cold eddy diameter of about100km in the spring of the Yellow Sea,and rotation transfer process may be nutrition "pump" of the seabed to the upper watercolumn for the growth of Ulva prolifera, which reveals the possible kinds ofspace-time dynamic process, also provides new clues to reveal the dynamic process ofthe outbreak of the green tide in the world within the scope of the entire biological-physical-chemical coupling.