A Study On The Infrared Imaging Nano - Probe And Visual Volume - Type Gel Sensor Based On

Positron emission tomography-computed tomography (PET-CT) plays an important role in molecular imaging technique. It has been widely used in life science researches, especially in clinical medicine, due to their high sensitivity. Some serious disease such as atherosclerosis, neurological disease, enteritis and diabetes come along with inflammation. The attempts to application of [18F]FDG as probe in PET imaging for the diagnose of inflammation has been achieved certain progress. However, for the most part, these advances have not translated into greatly improved clinical outcomes in some diseases. The reason for this is that [18F]FDG are lack of targeting to the immune response of macrophages and each [18F]FDG carried only one 18F atom which trapped the development of [18F]FDG probe as higher contrast agent. Furthermore, it is ignorant of a more effective targeted mechanism which is used for design and development of smart probe for inflammation.In addition, there is still other problem for radioactive probe, the leaking of radionuclide.18F labeled nanoparticle probe has received extensive attention for the researchers. It is supposed that positron-emitting radionuclide was doped in the process of synthesis of the nanoprobe. As a result, extremely large payloads of radionuclide were labeled to the nanoparticle, and more efficient signal enhancement as well as other advantages. Most importantly, the radionuclide was trapped in the nanoparticle avoiding leaking.Herein, we designed hydroxyapatite (HAp) nanoprobe by doping 18F-fluoride in the trace HAp nanoparticle in the process of synthesis. Due to the trace synthesis and doped method, the F-fluoride was not only on the surface but also in the inner. The HAp nanoprobe with extremely high payloads of 18F-fluoride was used for PET/CT imaging.In chapter one, the molecular imaging techniques, molecular probes, especially the PET/CT was introduced.The research work of the dissertation was made up of four sections:1. Synthesis of several nanoparticles with fluoride and NaYF4@SiO2@Au composite nanomaterials with core shell structures.(1) Synthesis of several nanoparticles with fluoride. Calcium fluoride, neodymium fluoride, lanthanum fluoride nanoparticles were facile produced by coprecipitation method with a short time. The morphology and particle size were characterized.(2) Synthesis of NaYF4@Si02@Au composite nanomaterials with core shell structures. NaYF4 nanoparticle was produced by coprecipitation method. Then the NaYF4@SiO2 was formed on the core of the NaYF4 with hydrolysis of tetraethoxysilane TEOS. The NaYF4@SiO2@Au was formed based on the strong coordination between the amino of NH3 and Au nanoparticles. It is expected that the core shell nanoparticle will act as multifunctional molecular imaging probes (PET/CT, MRI,01) and will widely applied to biomedicine due to the unique properties of Au nanoparticles.2.18F-fluorine doped HAp nanoparticles (18F-HAp) was prepared and the metabolism of the nanoparticles in the body of mice was studied.(1) 19F-fluoride labeled HAp nanoparticles were prepared in our lab and was characterized.(2) Base on the above test, the nanoprobe was designed with trace amounts of common fluoride labeled HAp, the same as 18F-fluoride. The stability of the nanoprobe in physiological conditions was studied.(3) 18F-fluoride from cyclotron labeled HAp nanoparticles as nanoprobe at hospital. Then the metabolism of the nanoprobe in the body of mice was studied, which can pave the way for PET/CT imaging.3. Synthesis of 18F labeled hydroxyapatite nanoprobe and the PET/CT imaging of rabbit.(1) In order to act as a ideal probe for PET/CT imaging, the nanoprobe with positron nuclide was reformed by means of funct’onalization.(2) The model of inflammation was established. The 18F labeled hydroxyapatite new nanoprobe was applied to PET/CT imaging of rabbit suffering from arthritis.4. The design and research of visual volumetric hydrogel sensor.(1) The preparation of fluoresce hydrogel. At first, Polyacrylamide (PAM) was produced according to the polymerization of acrylamide with deionized water and ethanediolglycol as solvent. Via Hoffman degradation, amide groups in PAM were partially converted into amine groups. Then,5,6-dicarboxylic fluorescein (5,6-DCF) with yellow green fluorescence was produced. At last, the hydrogel with yellow green fluorescence was synthesized through the dehydration between the amine group of aminated PAM and carboxylic group of 5,6-dicarboxylic fluorescein.(2) The preparation and application of visual volumetric hydrogel sensor. The hydrogel can undergo volume phase transition to Cu2+ ions, which enables a sensor with naked eye quantitative detection of Cu2+ by filling hydrogel into a graduated pipette with holes. The quantitative analysis of the copper ions can be accomplished by naked eyes. The character (selectivity, sensitivity, reproducibility, linear range) of the sensor was study. At finish, the sensor was applied to detect the environmental water sample.