Study On The Spectral Efficiencies Of Extracts From Schisandra Chinensis In Different Preparations

Schisandra chinensis is a traditional Chinese herb widely used to cure hepatic diseases. However, its effective compounds and quality standard have been not defined yet. The aims of the thesis were to study the relationship of "chemical component groups" of Schisandra chinensis defined by fingerprintology with pharmacodynamics. The present thesis was divided into three parts:i) To establish a method for the determination of six components of Schisandra chinensis together.High Performance Liquid Chromatography (HPLC) was adopted to determine the content of schizandrin, Schizandrol B, Schisantherrin A, Deoxyschisandrin, Schisandrin B, Wuweizisu C in the extracts of Schisandra chinensis. It can be used for quality control of six components of Schisandra chinensis as a simple and accurate method.ii) To construct the fingerprintology of Different Technics of Schisandra chinensis and study the its distribution and patterning:HPLC was adopted to analyse the components of different Technics of Schisandra chinensis, and to establish an HPLC digitized fingerprint for different Technics of Schisandra chinensis, when the correlative coefficient of comparability exceed 0.9, six components peaks is schizandrin, Schizandrol B, Schisantherrin A, Deoxyschisandrin, Schisandrin B, Wuweizisu C. Using cluster analysis by fingerprintology,7 different extracts were divided into 3 groups:a) Samples extracted by 90% ethanol,60% ethanol and methanol circumfluence; b) Samples prepared by 90% ethanol sonic extract, ethyl acetate circumfluence and CO2 supercritical fluid extraction; and c) samples prepared by 90% ethanol circumfluence with pulp.iii) To study the fingerprintology of Schisandra chinensis and its relationship with pharmacodynamicsThe protective effects of these different extracts of Schisandra chinensis on carbon tetrachloride (CCl4)-induced hepatic injury were observed in cultured hepatic cell and animal models. In cultured L-02 human normal hepatic cells, treatment with CC14 could induce a significant elevation of the activities of both alanine transaminase (ALT) and aspartate amino transferase (AST) and a marked increase in the content of lipid peroxidation product malondialdehyde (MDA) in cultured medium. However, co-incubation of the extracts of Schisandra chinensis (7 different extracts) could at some extents decrease CC14-induced lipid peroxidation and hepatic damage. In CCl4-induced acute Wistar rat hepatic damage model, the activities of both AST and ALT in plasma were significantly increased and the pathologic results showed the hepatic tissues widely necrosis and inflammatory cells infiltration. However, orally administration of the extracts of Schisandra chinensis (7 different extracts) could markedly decrease the activities of both AST and ALT and reduce hepatic tissue necrosis and inflammatory cells infiltration.The contribution of chemical component represent by fingerprintology to pharmacologic effects was analyzed using grey relational analysis. The results showed that the protective effects of Schisandra chinensis on CCl4-induced hepatic damage resulted from the actions of "different chemical component groups" each other. Based on grey relational analysis, the contribution ratio of chemical component on CC14-induced increase in AST activity of hepatic cell was:Schizandrin B> schizandrol A> Schisanthefin A> Schizandrol B> schizandrin A> Schizandrin C; the contribution ratio of chemical component on CCl4-induced increase in ALT activity of hepatic cell was:Schisanthefin A> Schizandrin B> schizandrol A> Schizandrol B> schizandrin A> Schizandrin C; the contribution ratio of chemical component on CC14-induced increase in MDA content of hepatic cell was:Schizandrin B> schizandrol A> schizandrol A> Schizandrol B> schizandrin A> Schizandrin C.The results of the present study suggest that all "effective chemical component groups" of Chinese herb contribute to the its pharmacologic actions, but the contribution ratio of each chemical component groups is different. The major findings of the present study were to establish the new method to evaluate the contribute ratio of "effective chemical component groups" to pharmacodynamics. The present study have theoretical implications and utility value, which play an exemplary role in the application of fingerprintology on Chinese herb industry.