Clinical And Experimental Study Of Shenlian Xiaozheng Tang On Medium Or Late Stage Primary Liver Cancer(PLC)

1. Objective:Observed the medium or late stage primary liver cancer on the rules ofsingle syndrom, in order to discuss the etiology and pathogenesis characteristic of thesouthern China PLC, offered the foundations for Shenlian Xiaozheng Tang (SLXZT); Toobserve the therapeutic efficacy of SLXZT on medium or late stage primary liver cancerand the basic efficacy on H₂₂ mice liver cancer, discuss the possible mechanism;2. Methods:2.1 Literature syudy: A literature review was carried out to summarize the westenmedcine and TCM etiology with pathogenesis of PLC,as well as its treatment. Meanwhile,based on the review results to discuss and expect.2.2 Clinical research: Collected 76 eligible patients from Gruangzhou and Jiangmen, toassess the single syndrome; 76 patients with stageâ…¡-â…¢liver cancer were dividedrandomly into two group, as SLXZT treated group A(42 patients) and control group B(34patients). The two groups accepted the same treatment with protecting liver, according tosyndroms and Sodium Cantharidinate Vitamin B6 Injection. Meanwhile, 42 patients inSLXZT treated group were treated with SLXZT more. The therapeutic efficancy of the twogroups were evaluated after curing one month.2.3 Experimental research:Copied the hepatocarcinoma 22(H₂₂) solid tumor-bearing micemodel, 30 male mice and 30 female mice were randomly divided into a normal group, amodel group, a CTX positive group, a low dosage of SLXZT, a moderate dosage of SLXZTand a high dosage of SLXZT, 10 mice in each group. The general condition, the inhibitoryrate of solid tumor and the thymus index were observed. T lymphocytes functions and thecell apoptosis was measured by Flow Cytometry(FCM). Radioimmunaoassay(RIA) examedthe content of IL-2 and TNF-αin serum.3.Results:3.1 Literature study: The results of literature review showed that the etiology withpathogenesis of PLC were not very clear. Generally comprehensive therapies are used totreat PLC. Multi-disciplinary comprehensive therapy combining with TCM has been the key to increase the curative effect and the hot topic of clinical study on cancer. In recentyears, some fruits on using integrated traditional and western medicine to treat PLC hasbeen achieved, which are not only about the mechanism of anti-cancer medicine but alsothe study on the curative effect.3.2 Clinical research:(1) The single syndrome distributing characteristics of the 76 patients were that spleendeficiency syndrome(65.79%), the most common syndrome in the patients.Othersyndromes in orders were Qi stagnation syndrome(51.32%), blood stasis syndrome(48.68%), excess of damp syndrome(40.79%), excess of heat syndrome(34.21%) andYin-deficiency syndrome(18.42%).(2) After curing one month, the two groups were better in symptoms thanpretherapy(P＜0.01).The Group A (54.76%)had a better effect in the remission ofsymptoms than Group B(29.41%), P＜0.05; The Group A (30.95%) had slightlyadvantage in improving the Kamofsky score than Group B (20.95%), but nosignificance in statistics(P＞0.05).(3) About stabilizing the tumor, The Group A (69.05%)had a same effect with GroupB(64.71%), P＞0.05; AFP still ascending after treatment, but there was no significantdifference among the two groups in the whole therapy course.(4) Comparing between the two groups, ALT, AST and TB descended than pretherapy, andthe descending of ALT has significant difference (P＜0.01);However, ALB and A/G alsodescended, but have no significance in statistics than pretherapy.(5) The results of peripheral blood T lymphocytes functions showed that the number ofCD3, CD4 cells and CD4/CD8 were significant increased in Group A than beforemedcine (P＜0.01) and have much better than Group B, CD8 had slightly decreased thanbefore medcine (P＞0.05).(6) There were no significant difference in blood analyse, kidney functions and cruorfunctions between the two groups among the whole therapy.3.3 Experimental research:(1) The effect of eliminating pathogenic factors:â‘ Mice models of the solid tumor livercancer were successfully established;â‘¡The tumor weights of each dosage of SLXZTgroup and CTX group were significant lower than model group, the inhibitory rate ontumor growth of CTX group was the lowest(45.27%). In the different dosage SLXZTgroups, the more dosage grew with the higher inhibition rate of minor.â‘¢The cellapoptotic rate of model group was 5.27%, each of the therapeutic groups wasstatistically higher than that of the model group (P＜0.01), CTX group was 12.25%. In the different dosage SLXZT groups, the more the dosage grew, the higher the cellapoptotic rate was.(2) The effect of strengthening anti-pathogenic qi:â‘ About general condition, the groupsdeal with SLXZT had less depilation phenomena and better activity than the CTX groupand model group;â‘¡The thymus index of CTX group was 27.29±6.03mg/g, whichstatistically lower than normal group (P＜0.05), each of the herb groups was higher thanCTX group, but have no significance in statistics;â‘¢The results of serum IL-2showed that compared with the normal group, IL-2 reduced in every experimentgroup,CTX group has the lowest IL-2 level, each of the herb groups was higher thanCTX group, serum IL-2 level of the high dosage SLXZT group was close to normalgroup;â‘£The content of serum TNF-αof every SLXZT group was higher than that ofthe normal group and CTX group, but lower than that of the model group;⑤Theresults of T lymphocytes functions showed that compared with the normal group,CD3,CD4,CD4/CD8 reduced in every experiment group, T lymphocytes functions wasfoul-up, SLXZT has the effect of increasing the content of CD3,CD4 and CD4/CD8.4. Conclusion:In the southern China, the special environmental and climate factors would influencethe etiology and pathogenesis of PLC. The single syndrome distributing characteristics ofthe 76 patients were that spleen deficiency syndrome(65.79%), the most common syndromein the patients.Other syndromes in orders were Qi stagnation syndrome(51.32%), bloodstasis syndrome (48.68%), excess of damp syndrome(40.79%), and the appearance oftheYin-deficiency syndrome(18.42%) was the least .According to the etiology andpathogenesis of PLC, which spleen deficiency is the principal aspect and qi stagnation andblood stasis, damp and toxic substances accumulation is the secondary aspect, ProfessorChen Ruishen established theapeutic methods was invigorate the spleen, replenishing qi,clear out damp, remove toxic substances, remove stasis and eliminate mass,named"Shenlian Xiaozheng Tang (SLXZT)". The results showed that SLXZT had a well effectin the remission of symptoms, in addition, it also had slightly advantage in improving theKamofsky score. SLXZT had a well effect on improving the cell-immunity.The results of experimental research showed that SLXZT had the effect of directkilling liver rumor in mice, inducing the apoptosis of the H22 liver cancer cells and wellimproving the immunity of mice,which would be the partial mechanism of SLXZT infighting the PLC.