A Study Of Molecular Epidemiological On Chronic Periodontitis In Hui And Dongxiang Minorities In Gansu

Purpose: In order To explore the association between the genetic polymorphisms and risk of chronic periodontitis, IL-1B gene single nucleotide polymorphisms (SNPs) at positions +3954, variable number tandem repeat (VNTR) polymorphism in IL-1RN gene intron 2, were examined and analyzed in the present work, based on the method of molecular epidemiology which provided scientific evidence for prevention, and to make early diagnosis of chronic periodontitis in the Hui and Dongxiang minorities in Gansu Province.Methods: A case-control study was conducted on 215 chronic periodontitis patients and 219 healthy controls from Linxia Hui and Dongxiang Autonomous Prefecture, Gansu Province. There was no statistical significance on age and gender between the two groups (P >0.25). The chronic periodontitis cases were choozen according to diagnostic criteria for chronic periodontitis recommended by Amitage. The DNA extracted from the blood samples of both groups was used for alleles' genotyping. Alleles on +3954 were genotyped with PCR-RFLP. IL-1RN VNTR polymorphism alleles were genotyped with PCR amplification and agarose gel electrophoresis analysis. Genotypes distributions were examined for significant departure from the Hardy-Weinberg equilibrium by a goodness of fit x~2 test. Odds ratio (OR) at 95% confidence interval (CI) was calculated to compare risk of the Chronic periodontitis between the examined genotypes. Chi squared analysis was used to examine differences in the proportions of the examined genotypes between different subgroups.Results: IL-1B +3954C/T heterozygote frequency (28.3%) in chronic periodontitis group was much higher than that in controls (15.8%), OR = 1.21 (95% CI 0.58-2.54), suggesting that IL-1B +3954C/T genotype was associated with the risk of chronic periodontitis in the Hui and Dongxiang population in the northwestern China and the haplotype of IL-1B +3954T was associated with a higher risk of chronic periodontitis. Based on the data from chronic periodontitis cases , it was suggested that allele A2 of IL-1RN were might increase chronic periodontitis risk in the studied Hui and Dongxiang population. The distribution pattern of gene mentioned above in Dongxiang is the same as that of Hui. The main possible reason was due to the similarity of the ethric sources and genetic background between Gansu Hui and Dongxiang minorities. In addition, they live in the same region and environment and bad(?) similar life habits.This study provided basic statistics for the distribution and categorization of the related susceptible gene of chronic periodontitis of Hui and Dongxiang minorities. It laid a foundation for the dynamic charges of the association between the succeptible gene and chronic periodontitis. It also provided a theoretical basis for the molecular categorization, diagnosis, and genetic theatment of chronic periodontitis.Conclusions: The present study revealed that, IL-1B +3954C/T genotypes were significantly associated with chronic periodontitis risk. It was suggested that allele A2 of IL-1RN were might increase chronic periodontitis risk in the studied Hui and Dongxiang population. The distribution pattern of gene mentioned above in Dongxiang is the same as that of Hui. The results indicated that genetic polymorphisms were also significantly associated with chronic periodontitis risk. The study data and results may provide scientific basis for prevention and early diagnosis of chronic periodontitis in the local population. The same kind of study on the Hui and Dongxiang ethnic groups has never been reported before in Chinese and foreign literature.