The Level Of Serum S100B And The Relationship Of Gene Polymorphism And Major Depression, And Sleep Electroencephalogram Physiological Characteristics In Patients With Major Depression

Objectives:(1) To detect the serum S100B concentration, to explore the clinicalsignificance of S100B in free-drug major depression, and to explorethe effect of different depressive episode numbers and gender on theserum S100B level in major depressive disorder (MDD)(2) To understand the distribution of glial-derive protein S100B genepolymorphism, to explore initially the relationship of S100B genepolymorphism and major depressive disorder. The purpose is tosuppose presumption as following: whether S100B may be thegenetic vulnerable factor in major depression.(3) To evaluate sleep clinical characters of major depression byPittsburgh Sleep Quality Index scale, and to detect multiplyingchannel sleep electroencephalogram, to explore sleep characterbetween first-episode and recurrent-episode depressive disease, tocompare the objective characters with subject characters in majordepression;(4) To evaluate Hamilton's depression scale (HAMD) and Hamiltonanxiety scale (HAMA) in depression, and investigate the relationshipbetween HAMD and PSG, between HAMA and PSG, between PSQIand PSG in depressed patients.Methods: (1) To adopt case control study, and 152 MDD were assessed withgeneral health state of oneself—developing questionnaire, HAMA,HAMD, 150 healthy controls were assessed with HAMD; 54 MDDpatients and 35 healthy controls were assessed with PSQI.(2) To measure the serum S 100B concentration in 54 depressed patientsand 35 healthy controls.(3) To detect the S100B gene polymorphism in 152 MDD patients and150 healthy controls by polymerase chain reaction—restrictionfragment length polymorphism.(4) To measure further the objective sleep indexes in 29 MDD patientsand 14 healthy controls by PSG equipment.Results:(1) There are significant differences in serum S100B levels in MDDpatients and controls (P＜0.05), and the serum S100B levels indepressed patients are higher than those in controls.(2) There is gender difference in depressed patients group, and the serumS100B levels in female patients are significantly higher than those inmale patients (P＜0.05). And There are significant differences inserum S100B levels between first-episode and recurrent- depressiveepisodes, between recurrent depressive disorders and normal controls(P＜0.05)(3) Serum S100B levels are significantly related with the numbers ofdepressive episode and depressive family history (P＜0.05).(4) There are not significant differences in the genotypic frequency andallele frequency of S100B gene rs9722C＞T or rs11911834G＞Tpolymorphism in depressed patients and normal controls (P＞0.05).there are significant differences in onset age and the subgroupdepression (first-episode and recurrent depression) in threegenotypes of S 100B rs9722C＞T in patients and controls (P＜0.05).there are significant differences in the subgroup depression (first-episode and recurrent depression) in three genotypes ofS100B rs11911834G＞Tin patients and controls (P＜0.05).(5) There are significant differences in sleep latency period, the awakenumber after sleep, RT percentage, REM latency, REM activity,REM intensity, the first REM sleep period (FRT), FRT percentage,REM number between depressed and normal controls (P＜0.05)(6) There are significant differences in S1 percentage, REM sleep time,RT percentage, REM sleep latency, REM density, FRT percentage infirst-episode and recurrent-episodes depression (P＜0.05).(7) There are significant differences in PSG variables (sleep latency,awake time, sleep efficiency, sleep maintenance, sleep stage 2, REMactivity, REM intensity, the first REM time, REM number) in maledepressed and female depressed patients (P＜0.05); PSQI total scores,sleep efficiency and sleep time are significantly related with the S2percentage, SWS, SWS percentage, REM activity in PSG indepressed patients (P＜0.05); PSQI total scores, sleep efficiency andsleep time are significantly related with the HAMA body anxietyscores, HAMA total scores, HAMD total scores, retarded scores,somato/anxiety scores, cognition disturbance scores in depressedpatients(P＜0.05); there are significant differences in sleep efficiency,actuality sleep time and sleep latency in objective sleep andsubjective sleep (P＜0.05). The sleep efficiency of subjective sleep isworse than those in objective sleep, practical sleep time of subjectivesleep is shorter than those of objective sleep, sleep latency ofsubjective sleep is longer than those in objective sleep.Conclusions:(1) S100B may be one of the indexes of functional disorder in brain;S100B involves the pathophysiology mechanism in depression.Different numbers have different pathophysiology mechanism inmajor depression. (2) S100B gene rs9722C＞T or rs11911834G＞T polymorphism are notsignificantly related with depression; there are certain differences indepressive episodes among different genotypes of S10B gene.(3) The depressed patients have severe sleep disorders.(4) There are significant differences in polysomnography variablies indifferent gender, different episode numbers of depression.(5) There are significant differences between objective sleep andsubjective sleep in depressed patients. Negativity cognitive indepressive patients deteriorates sleep results: they underestamatedtotal sleep time and overestimated sleep latency.