Monoclonal Antibody Study Of Differentiation Of The Odontogenic Epithelia

It is believed that proliferation of the rests of Malassez to form the cystic Lining and the junctional epithelium to form periodontal pockets are very important pathological processes during development of peri-apical and periodontal Lesions. However, little is known about cytokeratin expression of these peculiar epthelia and the changes of the expression associated with the proliferation.It has recently been demonstrated that there are about 19 kinds of cytokeratin peptides in human epithelium, and there appears to be a fundamental set of cytokeratins for a given epithelial differentiation. The pattern of cytokeratin expression within a particular epithelium may varies with its anatomy location, deve-lopmental stage and may also be affected by pathological processes. Therefore, examination of the changes in cytokeratin expression provides a useful way to study epithelial differentiation and, in some cases, assist pathological diagnosis.In present study; a panel of monoclonal antibodies to specific cytokeratins were used to demonstrated a: changes of cytokeratin expression asso(?)(?)ated with pro liferation of the rests of Malassez; b: patterns of cytokeratin expression of the epithelia in the dental follicles and odontogenic developmental cysts;c: cytokeratin phenotypes of junctional,sucular and oral epithelium of the gingiva; by means of immunocytochemical methods. Futhermore, it was tried to raise monoclonal antibodies by fusing; with Sp2 cell line, splenocytes of the rats immunized against lyophilized gingival tissue. In addition, immune cells in periapical granulo mata and odontogenic cysts were examined to colerate its patterns with associated epithelial elements.Results showed that cytokeratin 19 and 5 were main keratin components of the rests of Malassez and epithe-lial proliferation in periapical lesions were associated...