Effects Of CGRP On Cardiac Ca～(2+) Channel On Normal,Imitating Ischemia And Hypoxia Conditions And Simulation On Mathematical Model Of Cardiac Myocytes

Calcium Gene-Related Peptide (CGRP) is an important cardiovascular regulating peptide and has close connection with many cardiovascular diseases. In recent studies, CGRP showed its notable protective effects on arrhythmias. But there is still few reports on the effect. Especially the electrophysiological and ionic machenisms of this effect are unclear. Ischemia and reperfusion arrhythmias are very common with serious consequences on clinical manifestation and therapies for them are not satisfied. Therefore there comes urgency for finding an effective method to cure them. By using the patch clamp technique and laser scanning confocal technique, this study discussed the effects of CGRP on cardiac calcium channel on normal condition as well as on imitating ischemia and hypoxia conditions. And this study tried to discuss the electrophysiolodical ionic mechanisms of these effects so as to provide some theoretical and experimental proofs for wider clinical application. Meanwhile this study tried to reconstruct sodium, potassium and calcium channel dynamic courses and currents based on ventricular myocytes mathematical models and my personal experiments by using computer construction technique. The study could remedy the defect of animal experiments and open an effective way for providing theoretical proof for explaining the data from animal experiments by quantity study.The experimental designs were as follows:1. To grasp the culture technique of cardiomyocytes and study the direct...