Experimental Study On Preventing Acute Rejection In Rats Liver Transplantation Using NFκB-decoy Oligodeoxynucleotides

Part 1: The establishment of orthotopic liver transplantation in rats Objective To establish an ideal modal for orthotopic liver transplantation.Methods SD rats were used as experimental animals. Two-cuff (portal vein and infrahepatic vena cava) technique was applied in the establishment of orthotopic liver transplantation in rats. Survival time of recipient rats were analyzed and morphological investigations were studied on the grafts after liver transplantation.Results With the modification, a successful rate of 100% was achieved in the production of the experimental modal. Donor operating time was 25-35min; the grafts cold ischemic time was 40-55 min; recipients operating time was 45-65min with anhepatic period of 13-24min. Two week survival rate of recipients was 93%. Hepatocellular necrosis was observed on day 7-14 after liver transplanation and a large numbers of mononuclear cells infiltrating into the portal areas. On day 30 after liver transplantation, the parenchyma cells showed a nearly normal appearance, bile duct proliferation was seen in portal areas, and a few diploid hepatocytes were found.