| Despite more than a century of efforts have been taken to eradicate or control malaria, the disease remains a major and growing threat to the public health and economic development of countries in the tropical and subtropical regions of the world. Approximately 40% of the worlds population lives in areas where malaria is transmitted. There are an estimated 300~500 million cases and up to 2.7 million deaths from malaria each year, the mortality levels are greatest in sub-Saharan Africa, where children under 5 years of age account for 90% of all deaths due to malaria. The emergence and spread of drug-resistant parasites coupled with the absence of an effective vaccine makes malaria treatment more complicated, and thus the development of new antimalarial drugs and new vaccine targets is one of the urgent tasks in malaria research.The first object of mine was to evaluate the characterization of a novel gene, Pfdyn, which had been selected and predicted as candidate vaccine and drug potentiality by my group's previous works.By searching the NCBI malaria genomic database by tBLASTn, we find a putative protein in genome sequence which shares 38% homologous with dynamin protein. Although, it was evidenced by Southern Blotting that the gene had one copy in the parasite genome, no transcript was find in different asexual stages of the P.falciparum by Northern Blotting or RT-PCR. This suggests that Pfdyn may have different function in different intra-erythrocyte stages.The full ORF of Pydyn was amplified from mRNA of the erythrocytic stage P. yoelii, its full length was 2433 bp, and it encoded 811 amino acids which shares 75% homogenous with Pfdyn. This demonstrated that dynamin-like proteins were highly conserved in different Plasmodium species.We found some Pfdyn cDNA fragments in Plasmodium falciparum 3D7 gametocyte cDNA library and some Pfdyn peptide fragments in merozoite and gametocyte. So Pfdyn...
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