Study On Nootropic Mechanism Of Clausenamide

Clausenamide is a compound isolated from clausena lansium(lour) skella. (±), (+) and(-) clausenamide were synthesized firstly in our institute.In the previous study, Clausenamide was shown to improve consolidation of memory impaired by anoxia and NaNO₂. In this study, one way of passive avoidance response such as step down and step through tests and water maze test were used for comparison of effects of (±) clausenamide . ( - ) clausenamide and piracetam on learning and memory and memory impairment induced by anisodine and NaNO₃ in adult mice. Meanwhile, observation was also made for their effect on body and brain development in weaning mice.Computer-molecular modeling analyses was used to identify the nootropic potency of piracetam and (±) (-) clausenamide.The other methods and techniques used in present study include measurement of neurotransmitters, secondary messengers ( Ca²⁺, cAMP, cGMP, PKC, IP₈) , membrane ion pump (Na⁺K⁺ATPase, Ca²⁺Mg²⁺ATPase), ion channel, hippocampal slices potential, quantitative analysis of synapses and activity-dependent synaptic plasticity.—. Comparison of effects of piracetam and (±) , ( - ) clausenamide on learning and memory given by single or multiple doses in mice.Piracetam and (±), (-) clausenamide were given once or multiple dosage by i. p. or i. g. to mice for observation of their effects on learning and memory in the step- down and step-through and water-maze tests as well as their effect on amnesia impaired by anisodine and NaNO₂ . The results showed that piracetam, ( - ) clausenamide , (±)...