1. Experimental studies(1) It isindicated that electrolysis is themain killing action of electrochemical therapy (ECT) on esophageal carcinoma in vitro and MFC tumor of L615 mice in vivo. The ranges of PH are 1-2 in anode eraeand 13-14 in cathode area. The PH field formed around electrodes by various electricity dosages of ECT in ECA-109 cells culturewas invistigated. In vitro, when the electricity dosages were raised from 0.5 to 16.0C(6.0V, 3.8mA), the rate of the growth inhibition of ECA-109 cells was from 4.8 to 91.1% (p<0.05). The inhibition rate of mice tumor (MFC) growth ( D=1.0cm ) were 44.2% ( p<0.05 ) and 58.3% (p<0.05) when electricity dosages were 20 C and 40 C, respeclively; and suvival time of mice bearing tumor (MFC) with CET was 1.36 (p<0.05) and 1.61 (p<0.05), respeclively. The tumor was completely damaged in 80-100C.(2) ECT influenced of the permeability of ECA-109 cell membrane. In vitro a large amount of fluorescent Lucifer Yellow ( mw: 457.2 ) was induced into ECA-109 by ECT ( 6.0V, 3.8mA, 4.0C ), but not Rhodmamin Dextran ( mw:9000 ) . The fluorescent values by photometric measuremer in ECT group was 1.765±0.902, while in control group, 0.385±0.213 ( p< 0.05). And ADM could be induced by ECT into ECA-109 cells in vitro. The fluorescent values by cytophotometric analysis was 32. 52±18.29 in ECT+ADM group while it was 8.05 ± 4.09 in ADM group (p<0.01). The rates of growth inhibition of ECA-109 cells were 62.0% ( P<0.05 ) in ECT+ADM group (2. 0C and 0.5 ug/ml ), 14.0% in ECT group ( 2.0C ) and 11.2% in ADM group ( 0.5ug/ml ). The inhibitory rateoftumor of... |