Killing Effect And Induced Apoptosis On Lung Cancer Cells By Electrochemical Therapy (ECT)

To improve the effectiveness of Electrochemical Therapy (ECT) and to solve the problem of residual tumor cells in the treatment of tumors by ECT, the anti-tumor activity of ECT was studied, and the therapeutic of the treatment of lung carcinoma cells by low-level direct current was evaluated. In the mean while, the mechanisms of killing tumors of ECT were discussed.1. The killing action of Electrochemical Therapy on lung carcinoma in vitro and in vivoThe human lung squamous carcinoma cells L78 were culture in 24 well plates. The ECT (5 or 10 V, 0.5~4C) was performed by two platinum electrodes. 24 hours after treatment, the MTT assay was performed. The study in vivo was carried out in T739 mouse bearing LA795 mice lung adenocarcinoma. The tumors were treated by ECT (5V or 10V, 20C) when it grew to 1.0cm in diameter. In vitro, when the electricity dosage was 0.5 to 4C/well, the rate of the growth inhibition of L78 cells in group of 5V from 20%(P<0.01) to 80.70%(P<0.01). It was from 14.9%(P<0.01) to 83.28%(P<0.01) in group of 10V. As the electric quantity was less than 2C/well, the rate of the growth inhibition in group 5V was higher than that in group 10V. As the electric...