| By DNA-mediated transfection, two strains of human foreskin keratinocytes containing the HPV16 genome were obtained. These cells contained HPV16 DNA sequences and expressed HPV16 mRNA as demonstrated by Southern and Northern analysis. Both transformed cell strains showed much greater growth potential than the parental controls. They have been cultured in vitro for over 6 months and have reached passage 20 with no sign of senesce, suggesting that they are immortalized. They became feeder-independent and had a reduced capacity for terminal differentiation and stratification. In vitro transformation is a good model to study the interaction of HPV with human cells, and to investigate the process and mechanisms of human cell carcinogenesis.
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