Proteomic Studies Of Esophageal Squamous Cell Carcinomas

Esophageal squamous cell carcinoma (ESCC) is one of the common human malignancies. New cases of this disease increase by more than 300,000 every year. Because of late diagnose and poor therapeutic efficacy, five-year survival rate is lower than 10%. Increase of the long-term survival rate and reduction of the incidence rate depend on the understanding of the molecular mechanism of carcinogenesis. Previous researches mostly focused on isolating the esophageal cancer-related genes (oncogenes and anti-oncogenes). Although some advancement has been made, no gene specially associated with esophageal cancer has been found until now. there are still lack of early diagnoses markers and better therapeutic methods. In the study, proteomic techniques 2-DE and Mass Spectrum) were applied to analyze the diffferentially expressed proteins between the ESCC and adjacent normal epithelia.In order to minimize the influence of tissue heterogeneity to protein profiles, HE staining was used to locate the distributing of cancerous and epithelial cells under microscopy. Microdissection of routinely unstained frozen sections was used to procure cancer cells from esophageal squamous cell carcinomas and esophageal epithelial cells from normal tissues adjacent to the tumors. Through utilization of more effective surfactants, detergents and denaturants (CHAPS, Thiourea. TBP, Spermine) , the cells were lysised more completely and the dissolution of proteins was more sufficient,...