Study On The Mechanism Of Bisphenol A Developmental Neuronal Toxicity To Rat Male Offspring Central Dopaminergic Neuronal Pathway

objectiveMany animal behavior tests show that the central dopaminergic pathway may be a sensitive toxicity site by exposure to bisphenol A during brain development. The present study was designed to investigate the toxicity mechanism and effects of perinatal bisphenol A exposure on the central dopaminergic system development and function of rat male offspring.MethodsPart one Toxicity of bisphenol A on primary cultured fetal mesencephalic neuronal cell in vitro.1. To establish the in vitro serum free culture model of rat fetal mesencephalic neuronal cell. Mesencephalic neuronal cells were obtained from embryonic 14-15day SD fetuses and were seeded in serum contained medium for 24h first, then replaced by the serum free culture system of Neurobasal+B27. GFAP, NSE and TH immunocytochemistry was used to quantify the ratio of glial cell, neuronal cell and the dopaminergic neuronal cell on DIV4 and DIV7, respectively. The method and process was practiced and optimized for many times in order to have a relative highly and stabilized TH~+ cell ratio.