Study Of Detecting Agents Causing Sexually Transmitted Urogenital Infections By Gene Chip Techniques

An increasing prevalence of sexually transmitted urogenital infection (STUI) over the recent decades has become a profound effect on the health of human beings, especially of nongonococcal urethritis (NGU), because of its various kinds of pathogens, latent infection, and asymptomatic pathogen shedding.many studies have confirmed that STUI is commonly caused by the pathogens such as: Neisseria gonorrhoeae(NG), Trichomonas vaginalis(T. vaginalis), Chlamydia trachomatis(CT), Herpes simplex virus(HSV-1, HSV-2), Ureaplasma urealyticum(UU), Mycoplasma genitalium(MG), Mycoplasma hominis(MH), Candida albicans(C. albicans).The conventional methodologies, such as direct smear, bacterial or viral culture and immunoassay, are not only time-consuming and laborious but also lowly sensitive and accurate, have been continuously applied for detecting pathogens causing sexually transmitted urogenital infection (STUI) for many years as a result of absence of other substituted methods. Clinically and epidemiologically, it is necessary to develop a simple method with higher sensitivity in order to improve clinical diagnosis and epidemiological surveys. In view of this and through a systematic approach, i.e. reviewing relevant information/literatures, by combining with universal primer PCR and multiplex PCR techniques, STUI gene chip technique was developed to enable a single test to simultaneously detect various pathogens with a high specificity, sensitivity and accuracy.Chapter One: Design and Screening of Universal Primers, and Identification of Target Genes. First, we devided the target pathogens into three types: bacteria(Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis),viruses(HSV-1, HSV-2) and low eukaryotes( Trichomonas vaginalis, Candida albicans), Follwing the relevant references, the genes located at conservative regions of 9 pathogens, including DNA...