Research Of Gene Chip Techniques To Detect Agents Causing Genital Ulcer Disease

The conventional methodologies such as direct smear, bacterial or viral culture and immunoassay, although they are not only time-consuming and laborious but also lowly sensitive and accurate, have been continuously applied for detecting pathogens causing sexually transmitted genital ulcer diseases (GUDs) for many years as a result of absence of other substituted methods. Clinically and epidemiologically, it is necessary to develop a simple method with higher sensitivity in order to improve clinical diagnosis and epidemiological surveys. Since the gene amplification was available, a lot of laboratory tests based on such technologies to detect pathogens have been rapidly developed worldwide. These techniques, however, have still some limitations including the inability of detection of multiple agents simultaneously on one sample. In view of this and through a systematic approach, i. e. reviewing relevant information/literatures, definingnucleotide sequence of target genes, designing and screening the specific probes of various pathogens, and optimizing multiplex PCR conditions, GUD gene chip technique was developed to enable a single test to simultaneously detect various...