Effects Of GtfB Antisence Oligonucleotides On GTF-I Expression, Sucrose-dependent Adherence And Biofilm Formation Of Streptococcus Mutans

Antisenses oligonucleotides can regulate gene expression by binding to a complementary target by conventional Watson-Crick hydrogen bonds, thereby modulate the information transfer from the gene to protein. The inhibitor was designed directly according to the information of nucleic acid sequence. The inhibition of the targeted gene expression specifically depends on the base paring. Antisense oligonucleotides can be used to validate gene function and for therapeutic purpose. Antisense technology has been proved to be a simple, efficient and high throughput screening assay. Selective disruption and/or downregulation of gene expression are widely used tools for elucidating information of genes which are essential for bacteria growth, pathogenicity, and gene therapy. Antisense technology has been shown to be an effective means of controlling gene expression and has been used effectively to downregulate eukaryotic gene expression in a variety of systems. Although antisense regulation is a natural phenomenon in bacteria, it has not been routinely adapted for prokaryotes. A major challenge for the utilization of AS-ODN gene silencing technology is the inefficient entry of sufficient numbers of AS-ODNs into the targeted bacterial pathogen due to restrictions imposed by the outer...