Studies On The Protection And Immune Mechanism Of Oral Islet-cell Associated Antigens In NOD Mice.

Nonobese diabetic (NOD) mice spontaneously develop diabetes remarkably similar to human autoimmune insulin dependent diabetes mellitus. There is increasing evidence that human and the NOD disease result from immune destruction on the insulin-producing beta cells in the islets of Langerhans. The disease is characterized by progressive lymphocyte infiltration into the islets (insulitis) prior to the expression of overt diabetes and by the appearance of anti-islet cell antibodies in the serum. Oral administration of antigens has been proposed in the prevention and treatment of autoimmune disease.The aim of the study is: (1) to detect oral administration of recombination human insulin and glutematic acid decarboxylase (GAD) to NOD mice can prevent them from diabetes and insulitis. (2) to detect the effects of oral administration of insulin on apoptosis and Fas/Fas ligand expression on islet of Langerhans.(3) to characterize the mechanisms of active cellular suppression leading to diabetes prevention after oral insulin and GAD.Methods: (1) Oral antigen treatment: 96 female NOD mice were divided into three groups randomly. the first group was orally administrated recombination human insulin 1 mg in 500ul PBS (1.7 mM KH₂PO₄/5 mM Na₂HPO₄/150 mM NaCl) and the second one 500 ul PBS only at age of 4...