Endotoxemia And Bacteriemia In Surgical Patients

Objective (1)To observe the endotoxin levels in peripheral blood both in preoperative and postoperative samples. And analyze the relationship between endotoxin levels and complications / severity of trauma.(2) To evaluate the impact of alanyl-glutamine (Ala-Gin) dipeptide-enriched parenteral nutrition (PN) on clinical variables in postoperative patients, and investigate the effects of Ala-Gin on preventing bacterial translocation by detect bacterial DNA in peripheral blood. Background Bacterial translocation is thought to attribute to multiple organ dysfunction syndrome, but blood culture is often negative in these patients with clinical bacterial infectious manifestations. Endotoxin has been implicated in the processes that can lead to organ failure and death after surgery and critical illness. Depletion of Gln stores might lead to severe clinical complications. Recent studies indicate that the parenteral provision of Gln or Gin-containing dipeptides improves nitrogen balance, maintains the intracellular Gln pool, preserves intestinal permeability and absorption, and shortens hospital stay.Methods 50 healthy volunteers and 111 consecutive patients admitted to department of general surgery were enrolled in the study. Informed consent were obtained in all patients. Serial plasma endotoxin determinations were made using chromogenic limulus amebocyte lysate...