The Protective Effect Of Taurine On Isoprenaline-induced Myocardial Injury In Conscious Unstrained Rats

BACKGROUND Myocardial ischemia diseases are clinically characterized by repeated paroxysms of angina pectoris with unique electrocardiogram (ECG) changes. No animal model ideally fitted this condition the experiments to study effects of drug on ECG changes in animal suffering myocardial injury had to been conventionally carried out in anaesthetized animal. Because there is great discrepancy between narcosis and consciousness,as most of narcotics have significant actions on various systems, especially on cardiovascular and nervous systems, the results obtained in anaesthetized animal are usually poorly applicable to clinically conscious patients. Moreover narcotics may have synergism with myocardial injury drugs, and combination of them may cause irreversible injuries, and therefore, the results derived from anaesthetized animal are usually difficulty to replicate in the same animal. Consciousness and free moving would be ideal condition to use ECG to evaluate protective effects of drug in animal suffering myocardial injury. The DSI telemetric radio transmitter system (TA10CA-F40, Data Science International, DSI) makes it possible to record the ECG of conscious unrestrained rats. To obtain a repeatable ECG changes in conscious unrestrained rats and to simulate clinical situation of angina pectoris, isoprenaline (ISO) will be given repeatedly with proper interval and well adjusted dosage. A conscious unrestrained rat myocardial injury model with a repeatable ECG changes after each sc ISO would be an easier and more exact model for drug research. The effects of taurine (Tau) on this myocardial injury model will be studied.OBJECTIVEâ‘ To study the ECG changes induced by ISO and the relationship between the ECG changes and ISO doses, and to find out a proper ISO dose and a proper time interval to produce repeatable ECG changes after several successive administration of ISO in conscious unrestrained rats.â‘¡To compare the ECG changes obtained in conscious unrestrained rats with those...