Studies On Fingerprinting And Therapeutic Effect Against Rabbit Schistosomiasis Hepatic Fibrosis Of Angelica Sinensis And Compound Medicine Dangguibuxue Decoction

Traditional Chinese medicine (TCM) has a long therapeutic history and is widely used in the TCM treatment. Multiple constituents are usually responsible for its therapeutic effects. These multiple constituents may work 'synergistically' and could hardly be separated into individual active parts. In order to address the integrity and synergistic effects of TCM, the chromatographic fingerprinting was proposed as a technique to character its composition and efficacy, by the use of hyphenated instruments such as GC-MS, LC-DAD, CE-MS and LC-DAD-MS. The hyphenated analytical instruments could provide the additional spectral information, which will be very helpful for the qualitative analysis and even for the on-line structural elucidation. With the help of chemometric approaches, the hyphenated instruments provide us powerful tool for the analysis of complex mixture of TCM.The primary purpose of this work is to use hyphenated instruments and chemometric approaches to analyze TCM (single medicine and compound medicine). In all, this thesis covers four aspects as below.1. Qualitative and quantitative analysis of components in Angelica sinessis with hyphenated GC-MS and HPLC-DAD-MS methods (chapter 2 and chapter 3). Fingerprinting coupled with chemometrics HELP resolution is proposed as a tool for identifying and quantifying the volatilecomponents in Angelica sinensis during the plant growth period (chapter 2). HPLC-DAD-MS in different ionization mode is employed to characterize the chemical compounds in A. sinensis. By comparing the retention time, UV spectra, mass spectra and molecular weight of detected compounds with those published in literature, some phthalides of Angelica sinensis could be tentatively identified (chapter 3).2. Screening and Analysis of the Absorption and Metabolite Components in Rabbit plasma after Danggui Administration Using LC-DAD-MS (chapter 4). Based on chromatographic fingerprint technique, Combined approach of two-dimensional spectrochromatograms obtained from HPLC-DAD-MS and multicomponent spectral correlative chromatography (MSCC) algorithm was used to detect the absorption and metabolite components in rabbit plasma samples after Angelica sinensis administration.3. Relative adscription analysis of components between complex and single medicines of traditional Chinese medicines (chapter 5 and chapter 6). A compound medicine of Dangguibuxue decoction and its two involved single medicines of Danggui (Angelica sinensis) and Huangqi (Radix Astragali) were compared for their relevant constituents after the combination of the techniques of hyphenated chromatography and (multicomponent) spectral correlative chromatography, based on the two-dimensional spectrochromatographic fingerprints of the threemedicinal objects obtained from HPLC-DAD.4. Studies on therapeutic effect against schistosomiasis hepatic fibrosis in rabbits with compound medicine Dangguibuxue decoction and its single medicine (chapter 7). The rabbit models of hepatic fibrosis are obtained by intfection of schistosoma japonicum. After being treated with Praziquantel, the rabbits were divided into four groups: the first group was treated with Dangguibuxue decoction, the second with Danggui (Angelica sinensis), the third with Huangqi (Radix Astragali), and the forth with normal feed for three months. We evaluate the therapeutic effect of a rabbit model of schistosomiasis japonica Hepatic fibrosis using B-ultrasonic images. The degree of liver fibrosis treated with Dangguibuxue decoction is lower than those of the Danggui and Huangqi.