| Part I Organogenesis of rat metanephros anlagen after allo- and xenotransplantionIntroductionOrgan transplantation has become a well-established modality for treatment of end stage organfailure. The development of new immunosuppressive agents and UW solution, as well as the refinement of surgical techniques have accounted for the remarkable improvement in 1-year and 5-years patient survival, even long-term patient survival. However, almost all of patients undergone organ transplantation would receive permanent and regular treatment of immunosuppressive agents. For many patients with end-stage cardiac, respiratory or hepatic failure, the only chance ofsurvival is a transplant. In renal failure, although long-term dialysis is an option, transplantation offers better survival and quality of life as well as economic advantages. The major difficulty facing the transplant community currently is the shortage of organs for transplantation, and the complications caused by long-term administration of immunosuppressive agents.Transplantation of embryonic metanephros primordia may be a novel solution to these problems. Embryonic renal cellular primordia transplanted into animal hosts undergo nephrogenesis in situ, exhibit excretory function, and support life in otherwise anephric hosts. Renal primordia can be transplanted across isogeneic, allogeneic, and both concordant (rat to mouse) and highly disparate (pig to rodent) xenogeneic barriers.Here in this experimental section, we direct the aim to find out how can embryonic metanephros anlagen allo- or xenografted into hosts grow, defferentiate and undergo nephrogenesis and to evaluate the effect of Cyclosporine A(CsA) on the organogenesis and the function of embryonic metanephroi allo- or xenograft.Materials and MethodsMale and female rats of the same strain were mated overnight, and female rats were scored for vaginal plaques in the next morning. The presence of vaginal plaques was taken to represent embryonic day 0(E0). Whole fetal metanephroi from embryonic day 14 to 19 (E14-E19) of Sprague-Dawley (SD) or E15 Lewis rats were used to procure kidney anlagen to perform the present experiment. Organogenesis of developing metanephroi allografted into nonimmunosuppressed adult ratsWhole metanephroi from embryonic day 14 to 19(E14-E19) of Sprague-Dawley (SD) were grouped and allografted into the omenta of nonimmunosuppressed (E14SD, E15SD, E16SD, E17SD, E18SD, E19SD) SD adult rats (E16CsASD, E17CsASD) which had one kidney resected, or host rats with both naive kidneys intact served as controls. By 4 weeks after implantation, metanephroi allografted in host rats were removed for histopathological examination or anastomosed for renal function measurement 4 weeks later.Allograft of developing metanephroi into CycIosporine(CsA)-treated adult ratsWhole metanephroi from embryonic day 15,16 and 17(E15, E16, E17) of SD rat were grouped and allografted into the omenta of CsA-treated (E15CsASD, E16CsASD, E17CsASD) or untreated(E15SD, E16SD, E17SD) SD adult rats; E15 of Lewis(RT1~1) metanephroi grouped (E15CsABN and E15BN) and grafted into Brown Norway (BN) (RT1~n) adult rats, which had one kidney resected. 10 rats of each group were studied, and received daily sc injection of CsA (8mg/kg/d) or equal volume of saline; By 2 to 4 weeks after implantation, metanephroi allografted in host rats were removed for histopathological examination or anastomosed for renal function measurement 4 weeks later. Xenograft of rat developing metanephroi into adult miceWhole metanephroi from embryonic day 14,15 and 16(E14, E15, E16) of Lewis (RT1~1) metanephroi were grouped and grafted into C57BL/6 (H-2~b) adult mice, which had one kidney resected. 10 mice of each group were studied, and received daily sc injection of CsA (12mg/kg/d). By 2 weeks after implantation, metanephroi allografted in host mice were removed for gross and histopathological examination.ResultsOrganogenesis of developing metanephroi allografted into nonimmunosuppressed adult ratsFour weeks post-implantation, E19SD and E18SD metanephroi had...
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