Study Of Loss Of Heterozygosity And Mutation As Well As Influencing Factors Of Expression Of ING1 In Human Gliomas

Gliomas is the most common primary intracranial tumor with poor prognosis and high mortality rate. There is no progress in treatment strategy and effect for many years. In this study, we examine heredity change of 45 human gliomas biopsy in order to provide new keys to pathogenesis and genetic treatment methods of gliomas.We select five human microsatellite makers to examine loss of heterozygosity of ING 1 in gliomas by using the amplified fragment length polymorphism and filtrate possible gene mutation by using single strand conformation polymorphism analysis of polymerase chain reaction products. The results show that there are no loss of heterozygosity and gene mutation in all 45 gliomas.The result indicate that down regulation of ING1 expression may be due to some upstream control mechanism which leading to abnormal in or after transcription. We presume that telomere and telomerase may be one of the upstream control factors.