Analysis Of Relevant Factors Related To Postoperative Laryngeal Cancer Recurrence

Clinical ResearchClinical analysis of relevant factors causing postoperative laryngeal cancer recurrenceObjective To investigate the clinical relevant factors causing postoperative laryngeal cancer recurrence and to analyse the reasons of treatment failure. Methods Seven hundred and fourty-five cases of laryngeal cancer from 1958 to 1999 treated in ENT Department of PL A General Hospital were reviewed retrospectively. Among them, 574 cases were followed up for more than 3 years postoperatively, 171 cases were lost and 87 cases died. Totally, 56 cases of the recurrence were diagnosed. The possible factors related to the recurrence including: age, sex, history, admission time in hospital, smoking, grade of pathology, complications, stage of tumor, blood transfusion, radiotherapy, operation procedures, cervical lymph node metastasis. Results The statistic analysis showed that stage of tumor (.P=0.0002) , operation procedures (/>=0.0017) , blood transfusion (P=0.0001) , radiotherapy (P=0.0001) , cervical lymphatic node metastasis (P=0.0228) were significant for postoperative laryngeal cancer recurrence. Conclusion Stage of tumor,blood transfusion, radiotherapy, operation procedures, cervical lymph node metastasis were significant risk factors which related to postoperative laryngeal cancer recurrence.Basic ResearchExperiment 1 Significance of p53, c-myc and nm23 protein expressions in tissue arrays of patients with recurrent laryngeal carcinoma.Objective: To evaluate the prognosis of postoperative laryngeal cancer recurrence, the study was designed to investigate the relationship of p53, c-myc and nm23 protein expression with recurrent laryngeal carcinoma. Methods: 574 cases of laryngeal carcinoma treated in our department from 1958 to 1999 were retrospectively reviewed. All patients were observed for 3 years postoperatively. The whole set of tissue microarrays contained tumor tissue from 574 patients, including recurrent tumor tissue from 56 patients, 39 normal laryngeal tissue from normal laryngeal. The tissue chips were analyzed by the SABC immunohistochemical method. Results: p53, c-myc and nm23 were expressed in most of laryngeal carcinoma specimens. The positive ratio was different. There was higher expression of c-myc in recurrent laryngeal carcinoma compared to that in laryngeal carcinoma without recurrence, the difference was significant (P<0.001). There was lower expression of nm23 in recurrent laryngeal carcinoma compared to that in laryngeal carcinoma without recurrence, the difference was significant (PO.001). There was no significant difference of p53 expression between laryngeal cancer groups with, and without recurrence (P>0.05). There was positive correlation between the expression of c-myc and the recurrent laryngeal carcinoma; and negative correlation between the expression of nm23 and the recurrent laryngeal carcinoma. Conclusion: c-myc and nm23 were the prognostic marker candidates for the postoperative laryngeal cancerrecurrence.Experiment 2 Significance of Cyclin-Dl, PCNA, and ki-67 protein expressions in tissue arrays of patients with recurrent laryngeal carcinoma.Objective: To evaluate the prognosis of laryngeal cancer postoperative recurrence, the study was designed to investigate the relationship of Cyclin-Dl, PCNA, and ki-67 protein expression in patients with recurrent laryngeal carcinoma. Methods: Same as experiment 1. Results: Cyclin-Dl, PCNA, and ki-67 were expressed in most of the laryngeal carcinoma and normal laryngeal tissue. The positive ratio was different. There was higher expression of Cyclin-Dl, PCNA, and ki-67 in recurrent laryngeal carcinoma compared to that in laryngeal carcinoma without recurrence and normal laryngeal tissue (P<0.001). There was positive correlation between the expression of Cyclin-Dl, PCNA, and ki-67 and the recurrent laryngeal carcinoma. There was also significant difference between the laryngeal carcinoma and normal laryngeal (P<0.001). Conclusion: Cyclin-Dl, PCNA, and ki-67 were the prognostic marker candidates for...