A Study On The Mechanism Of "Sanzi Yang Yang Tang" To Improve Asthma Symptoms By Inhibiting CysLTs Mediated Inflammatory Pathways

Objective:To explore the Mechanism of Three sons adoptive parents soup inhibition the CysLTs mediated inflammation pathway, and on this basis, To observe and evaluate the effect of Three sons adoptive parents soup in the treatment of asthma due to wind and phlegme。Methods:Under the guidance of TCM theory’ treat asthma must first treat shortness of breath", to "From the clinical view, eventually returned to the clinical" evidence-based research-oriented thinking as a guide. Set up Three sons adoptive parents soup as the research object, the establishment of an animal model of asthma, By PCR biotechnology,research the Three sons adoptive parents soup Influence of the expression in CysLT1mRNA in asthmatic animals and regulation of IL-4, IL-5 and other inflammatory mediators, Inflammatory cells in bronchoalveolar lavage fluid and lung histopathology. Finally, to validate its mechanism in the body, To observe the total efficiency, TCM syndrome integral, bronchial pulmonary function test, dynamic changes in albuterol usage, the levels of total IgE in serum, EOS%, IL-4, IL-5 content in sputum, leukotriene E4 in urinary and other indicators of Three sons adoptive parents soup in treating Asthma patients.Result:In animal experiments, SZ-M group can significantly increase the autonomy of sport, food intake and body weight in asthmatic mice, Three sons adoptive parents soup can reduce the content of neutrophils, eosinophils, white blood cells and other inflammatory cells in bronchoalveolar lavage fluid of asthmatic mices, the most significant is SZ-M group.The IL-4, IL-5 content in bronchoalveolar lavage fluid is significantly higher than in normal mice. SZ-M group can significantly reduce the IL-4, IL-5 content, Three sons adoptive parents soup can downregulated the expression of CysLT1 mRNA in lung tissue, the most significant is SZ-M group and SZ-H group. hree sons adoptive parents soup can make better run inflammation and tissue edema sexual assault in lung tissue of asthmatic mice, the most significant is SZ-H group. In clinical observation, Three sons adoptive parents soup have high total efficiency, Can significantly improving the symptom scores of asthma due to wind and phlegme, Can effectively reducing the amount of albuterol usage, improving in the bronchial pulmonary function test, Three sons adoptive parents soup have a considerable effect with montelukast, While reducing the eosinophil infiltration, Inhibiting the release of IL-4, IL-5 and other inflammatory factors, And reducing the content of urinary LTE4 and serum total IgE, Three sons adoptive parents soup haven t appear the significant adverse reactions.Conclusion:As can be seen from animal experiments, Three sons adoptive parents soup can inhibit CysLTs mediated inflammatory pathways and Inhibit the expression and release of inflammatory cytokines, thereby reducing airway inflammation; As can be seen from the clinical observation,Three sons adoptive parents soup in the treatment of asthma which belongs to wind and phlegme have Significant clinical effect, and fewer adverse reactions, worthy of clinical application.