Differential Expression Of Retrotransposon WIS 2-1A In Wheat Response To Low-energy N⁺ Implantation And ⁶⁰Coγ-ray Irradiation

Differential expression of retrotransposon WIS 2-1 A in wheatresponse to low-energy N⁺ implantation and ⁶⁰Coγ-ray irradiationPurpose: This paper tried to investigate the variation of retrotransposon WIS 2-1Aactivity in wheat (Triticum aestivum L.) embryos at three different developmentalstages (30 h, 45 h and 60 h) after treating with 0.5×10¹⁷,1.0×10¹⁷,1.5×10¹⁷ N⁺/cm²implantation and 100Gy, 300Gy, 500Gy ⁶⁰Coγ-ray irradiation respectively. Wediscussed the relationship between the expression of WIS2-1A and the variable dose ofN⁺ and ⁶⁰Coγ-ray from the level of DNA and cDNA. What's more, we tried to find theinfluence of the high expression of WIS2-1A to the whole other transcripts using thewheat genome array. This paper could promote the theory of radiant aberrance.Results:1 .The DNA composition changed a little of WIS2-1A reverse transcriptase aftersufferring N⁺ implantation and ⁶⁰Coγ-ray irradiation.2. There is a vacancy of 9bp in the sequence of W1S2-1A reverse transcriptase aftersufferring 1.0×10¹⁷ N⁺/cm² implantation. It's translation product has 6 terminators andthat probably result the heterogeneity.3. WIS 2-1A always had the transcription activity in the first 60 h of incubation and areverse relationship between the time of embryo development and the sensitivity ofretrotransposon WIS 2-1A. For N⁺ the expression showed a regular fluctuation withthe gradual doses. The most positive stimulation occurred at the dose of 1.5×10¹⁷N⁺/cm² at 30 h. For ⁶⁰Coγ-ray the expression showed a irregular fluctuation and theexpression changed a little.4. The membrane permeability, respiration, synthesis of carbohydrates and proteinincreased, but the hydrolysis of carbohydrates and protein slowed down. The proteinof resisting low-temperature, high temperature and stress increased after 1.5×10¹⁷N⁺/cm² implantation at 30 h. Low-energy N⁺ implantation caused some injury towheat embryo cells, after 30h culture, the cells are in the active state of stress adaptation.5.comparing the control,the expression of WIS2-1A and some resistance geneincreased greatly. It seems like that they have the same mechanism to regard the cells.