Study On Genetic Diversity And Phylogenetic Relationships In Puionium Gaertn

Puionium Gaertn , biennial plants in cruciferae, including P.cornutum (L.) Gaertn.and P.dolabratum Maxim, were versatile sandy vegetable, which were also used for herbs, feeding and binding-sand, they mainly distributed in desert in Mongolian plateau. Nurtritional components of leaves, morphological characteristics of cotyledon epidermiswere, genetic diversity and phylogenetic relationship were investigated in P.Gaertn. were estimated or investtigated in P.Gaertn., and phylogenetci relationship between P.Gaertn and several genera in cruciferae was estimated. The main results showed that:1. Four new germplasms were found in the review and collected of germplasm resource, which enriched the germplames of P. Gaertn.2. There were differences in nutritional components of leaves between P.cornutum(L.)Gaertn.and P.dolabratum Maxim, for example Vitamin and amino acid.3. Morphological characteristics of cotyledon epidermis of P.Gaertn. were investigated by using scanning electron microscope (SEM), Bulliform cell, the endemic structure in Graminaceous, was firstly found in cotyledon epidermis of P.Gaertn , and there were abubandce diversity in cotyledon epidermis in P. Gaertn.4. The DNA extracted by the modified method of CTAB, can completely meet with the experiments related to SSR and SRAP marker, the reaction of SSR and SRAP of P.cornutum (L.) Gaertn. were establishee and optimized, which were steady and reliable.(1) SSR reaction system:1.5mmol/L, Mg2+, 0.2mmol/L, dNTPs, 0.4μmol/L primer, 1U Taq polymerase, 20 to 30ng template DNA were the optimal conditions in the reaction of 10μL volume;(2) SRAP reaction system:1.5 mmol/L Mg2+, 0.2 mmol/L dNTPs, 0.4μmol/L primer, 0.3 U Taq polymerase, 70 ng template DNA were the optimal conditions in the reaction of SRAP 10μL volume.5. Transferbility of Brassica.napus EST-SSR marker to P. Gaertn showed that the amplification percentage of tansferbility were relatively 1.96% and 1.5% in P.cornutum (L.) Gaertn. and P.dolabratum Maxim.6. Analysis of genetic diversity SSR and SRAP of 7 populations in P.cornutum (L.) Gaertn(1) Analysis of genetic diversity SSR of 7 populations in P.cornutum (L.) Gaertn40 loci were detected in 10 SSR primers, polymorphic loci percent were 90%; the percentage of polymorphic loci ranged from 40.00% to 62.50%; Nei's gene diversity ranged from 0.1230 to 0.2109,shannon's Information index ranged from 0.1872 to 0.3162 in population; There were 71.63% genetic variance within population and 28.36% among populations.(2) Analysis of genetic diversity SRAP of 7 populations in P.cornutum (L.) Gaertn138 loci were detected in 14 SRAP primers, polymorphic loci percent were 92.7 %; the percentage of polymorphic loci ranged from 56.20% to 71.53%; Nei's gene diversity ranged from 0.2036 to 0.2338, shannon's Information index ranged from 0.2999 to 0.3589; there was 70.82% genetic variance within population and 29.18% among populations.The results of two makers dicated genetic diversity was low in populations.7. Results of genetic diversity and phylogenetci relationship SSR and SRAP analysis in P.Gaertn showed that:(1) Results of genetic diversity and relationship SSR analysis in P.Gaertn45 loci were detected in 7 SSR primers, polymorphic loci percent were 90%; the percentage of polymorphic loci ranged from 43.90% to 65.85%; Nei's gene diversity ranged from 0.1370 to 0.1918, shannon's Information index ranged from 0.2173 to 0.2957,there was 75.18% genetic variance within specie or types and 24.82% among specie or type.(2)Results of genetic diversity SRAP analysis in P.dolabratum Maxim.157 loci were detected in 19 SRAP primers, polymorphic loci percent was 90%, the percentage of polymorphic loci ranged from 61.15% to 76.43%; Nei's gene diversity ranged from 0.1966 to 0.2513,shannon's Information index ranged from 0.3017 to 0.33792; there were 80.18% genetic variance within type and 19.82% among type.The genetic diversity were lower in P.cornutum (L.) Gaertn than P.dolabratum Maxim. The gradation of genetic diversity from high to low was special type, kuan chi type, ju guo type, chi guan type and fu xing type.8. Resuls of genetic relationship SRAP analysis between P.Gaertn and other genera in cruciferae showed that: the gentic relationship was far among P.Gaertn., B.Rapa, B.napus, B.olevalec, B.carinata, B.juncea B.nigra, Raphanus sativus.L, Isatis indigotica Orychragmus violaceus O.E.Schulz.