Studies On The Protoplast Culture And The System Of Somatic Cell Fusion Of Vitis Davidii Fo(?)x

Vitis davidii Foex is an eastern Asia wild resource that belongs to Vitaceae family . Its natural range is in Shianxi,southern Gansu, Yunnan-Guizhou Plateau and the Yangtze River, and often distributes in the valley below the altitude of 1500m or beside the woods edge. Vitis davidii Foex is a vigorous plant with rough leaves , thick shoots with upright or slightly curve peel thorns , long tassel (above 20cm), big fruit (15mm or more in diametre) which is tasteless and late-ripen , thickness pericarp , high yield and fewer disease and pests. It is resistant to white rot , anthrax and sphacelma ampelinum, but receptive to downy mildew. Two excellent cultivars were selected for fresh fruit from Vitis davidii Foex: 'Tang Wei' in Jiangxi and 'purple autumn' in Hunan Agricultural University. Vitis davidii Foex is tolerant to humidity and shade. It is usually increased vegetatively from grafting , taken Vitis pseudoretiaculata as stock , not from cutting . Vitis davidii Foex is a idea germplasm resources for breeding heat resistance , humidity resistance and disease resistance ; and may also be used as stock.This paper using Vitis davidii Foex as material studied the callus inducement, in vitro culture and micro-propagation , protoplast isolation, protoplast regeneration. In order to provide scientific basis and operating techniques for Vitis davidii Foex multiplication and disease resistance breeding, we used Vitis davidii Foex and its cultivar 'Muscat Hamburg' as materials to study protoplast electrofusion.1 .Callus inducement from explants of Vitis davidii FoexTo study the callus inducement rate and subculture of the Vitis davidii Foex , the stem segment, tendril, leaf, young fruit and carpopodium were used as the explants.on medium 1/2MS and B₅ containing various growth regulators, and callus was trafferred to B5 with various growth regulators were study the shoot regeneration, micro-propagation and rooting of Vitis davidii Foex. The results showed that optimum basic medium for inducing callus were 1/2MS and B₅ . Oppositely dissected stem segment was the best induction material. The optimal medium for callus induction was B₅+2,4-D3 mg·L^-1+BA0.01 mg·L^-1 with the highest induction rate up to 100%, and the quality of the callus was high. The optimum medium for subculture was B₅+2,4-D0.5 mg·L^-1 +6-BA0.2 mg·L^-1, subcultured once in 4 weeks; the density of callus...