Studies On Genetic Transformation Of Chrysanthemum Morifolium With PEAMT Gene Mediated By Agrobacterium Tumefaciens

Chrysanthemum morifolium is one of the most famous flowers in China and ornamental species in the world. Ground-cover Chrysanthemum is a superior breeding group of Chrysanthemum morifolium, which applies to land growth in gardens with plenty of colors, wide coverage and excellent ornamental value. However, the application of Ground-cover Chrysanthemum is restricted by the booming problem of lacking water resources and salification in urban areas. Agrobacterium tumefaciens taken as the transformation vector, the spinach's PEAMT gene, which catalyzes the synthesis of glycine betaine, is transferred into the genome of Ground-cover Chrysanthemum. Therefore, this transfer will result in the accumulation of compatible solute--glycine betaine and the enhancement of tolerance in transgenic plants under the stress conditions of drought, salt and low temperature. Furthermore, the transgenic Ground-cover Chrysanthemum can be widely applied into use around the country and serve the gardens.The main research work and results were as follows:1. The genetic transformation receptor systems for'Yurenmian'and'Pudijin'were established. Using 9 different concentration proportion of 6-BA and NAA which were based on MS culture medium, 3 high efficiently shoot regenerating cultivars, that was'Yurenmian','Pudijin'and'Beilinhong'were selected from 10 cultivars of Ground-cover Chrysanthemum, with the regenerating rate being 98%, 96% and 96%. The optimal regeneration medium for'Yurenmian'was MS+6-BA1mg/L +NAA0.1mg/L, for'Pudijin'was MS+6-BA2mg/L +NAA1mg/L and for'Beilinhong'was MS+6-BA1mg/L +NAA0.5mg/L. At the same time, the factors which would influence shoot regeneration such as the different explant types, timing culture in vitro were studied. The results showed that: the leaves'regeneration ability was best, timing culture in vitro influenced the leaves'regeneration ability and the time of keeping high...