Candidate Genes Research For Prolificacy In Partial Chinese Goat Breeds

Most goats in china have long-term of pre-production raising period and low economical contribution. Upgrading their productive characteristics, through improving prolificacy could be the most effective method. Most goat breeds in the world have seasonsal estrus and low rate of multi-fetus, however, Chinese nining grey goats are non-seasonal estrus and have a high rate of multi-fetus. In this research, mRNA differential display method and candidate gene method were used to perform a primary screen to discover some ESTs and candidate genes, which may affect the molecular genetic mechanism of high fertility of Jining grey goats to some extent, and to offer some theoretical evidences for controlling the reproductive traits of goats from genetic method, to improve the prolific capability of goats.In DDRT-PCR part, 1195 cDNA fragments were found, and 24 differential expression bands were re-amplified. Blast software was applied to align and analyze the data after cloning and sequencing. The results indicated that 10 differential expression bands were aligned to YidC gene, GATA transcription factor and CAT gene, and 14 differential expression bands have not obtained the homologous sequences or have obtained the homologous sequences but the function of the sequence was still unknown. The conclusions of this part were:(1) GATA transcription factor has obviously function in goat reproductive system, and has an effect on goat sexual decision and reproduction regulatory.(2) YidC gene may relate to transport of goat reproduction cell.In the candidate gene method part, the special site or partial exon of four genes were detected by PCR-SSCP or PCR-RFLP method in Jining grey goats, Boar goats, Liaoning cashmere goats, Inner Mongolia cashmere goats, Wengden milk goats, and Beijing native goats. The genes were 1184 site mutation(C1184T) of GDF9 gene, 718 site mutation(C718T) and 1100 site mutation(G1100T) of BMP15 gene, most of exon sequence of MTNR1A gene and 5'-untranslated sequence and exon 1 of INHαgene.