Isolation Of OsARGOS Gene And Function Analysis In Oryza Sativa

Lateral organs that have different morphology, structures and functions are produced during plant life cycles and morphogenesis. They are single lateral organs derived from meristemic regions. Organ size is the most obviously different among the same organs in different plant species. Because of their immovability and light-dependent lifestyle,organ size of plants is frequently influenced by external environmental signals. Nevertheless, there are a lot of evidences that organ size is primarily controlled by internal developmental signals in plants. Analysis of mutants and identification of related genes on lateral organ size provided important clues to understand the molecular mechanism of the control of organ size. Previous studies on the control of organ size are mainly focused on the dicots. But little is known about the monocots because of limited available mutants. Rice is one of the important cereals in the world and a model plant species in the monocots. The molecular mechanism of the control of organ size in this crop still remains blurry. In this study, we isolated the gene OsARGOS from the rice leaves and further analyzed its functions. Our data may provide some information on the molecular mechanism of the control of organ size in plants.1. Isolation and characterization of OsARGOSTo investigate the molecular mechanism of the control of organ size in rice, we isolated cDNAs of OsARGOS from the leaves. The cDNA is 596 bp enconding a protein with 178 amino acid residues. This gene localizes in the Chromosome 4 in rice. The protein contains a Leu-rich domain at its C terminus. BLAST analysis indicates the gene OsARGOS is the homolog of Arabidopsis ARGOS.2. Localization of OsARGOS protein in the cell...