| A ccording to some problems of in vitro rooting difficult in virus free and in vitro multiplication of some grape cultivars, some important effects of in vitro microenvironment, light, temperature, pH value and compositions of culture media on the rooting in vitro of grape test-tube plantlets, the content changes of endogenous hormones and endogenous metal elements, and the differences of endogenous materials and biological effects of DCP (Di-n-cotyl pthalate)of grape test-tube plantlets of different in vitro rooting abilities during multiplication were studied in this dissertation. The conclusions were drawn as follows:1. Shifuluosha and Sentianni seedless easy to root, and Fujiminori and Huangjiaqiutian hard to root were selected as test materials. When the sucrose concentration in the media was changed from 5g/l to 25g/l in turn, the root initiation of grape test-tube plantlets of 4 cultivars was prolonged gradually, but root initiation at 20g/l was the slowest than at 25g/l. The changes of sucrose concentration from 5g/l to 20g/l, the root numbers were increased with the increase of the sucrose concentration, but decreased afterwards in different cultivars and treatments, and the differences were significant. Average root length and R/T(root/tip) rate of test-tube plantlets differed significantly among the different treatments, and the optimum concentration of sucrose was 15g/l. It indicated that the different concentrations of sucrose played some important roles in in vitro rooting of grape test-tube plantlets. The conclusion was drawn that the concentration changes of sucrose between 15g/l and 20g/l were optimum to root in vitro.2. The effects of different pH values on the average number of root, the average diameter of root, the average length of each root and average R/T rate were significantly different. With the increase of pH value from 5.0 to 7.0, the average number of root and the average length of each root decreased, but the average diameter of root and the average R/T rate increased. The differences of 4 indexes above were significantly different at 0.01 level.3. The changes of agar concentrations in the media from 3g/l to 7g/l, the average initiation time of test-tube plantlets of 4 grape cultivars was prolonged, and the average length of roots decreased, but instead of the average diameter of roots. The differences of 3 indexes above in different treatments were significantly different. The average root numbers of Shifuluosha, Sengtianni seedless and Huangjiaqiutian and the average root diameter of Fujiminori increased with the increase of the agar concentrations, and the optimum concentrations were 6.0g/l and 7.0g/l, individually. The differences of all treatments were significantly different.4. The rooting number of grape test-tube plantlets treated at 20,25 and 30 had no significant difference, but it was inhibited definitely at 35,however, other treatments were significantly different at 0.01 level. With the temperature increased, the average diameter of root was decreased a little, but this change was not obviously. Under 20,25 and 30, the average length of each root was increased with the temperature increased, but it was minimum at 35. The average R/T rate was increased with the temperature increased. At 35, the R/T rate was significant higher at 0.01 level than those of other temperatures , but the differences among 20,25 and 30 were not significant. So, the conclusion of this experiment was consistent with grape micropropagation in vitro, the optimum temperature was 28 2 .5. The number of roots, the average length of each root, the average R/T rate and the average diameter of root of grape test-tube plantlets of 4 cultivars under three different light intensities, 30001x, 20001x and 5001x were increased with the increase of light intensity. It was consistent with the light intensity of 20001x used usually in the plant tissue culture .6. Four grape plantlets had not root in vitro from the 1st day to the 5th day after inoculating, under the six kinds of light conditi... |
PDF Full Text Request