Design, Synthesis, Supramolecular Recognition And Interaction With DNA Of Macrocyclic Polyamine Complexes Which Containing Nucleobases, Polyamides

Artificialnucleases have been proven to be efficient tools for the footprinting and sequence-specific targeting of nucleic acids. The development of reagents which hydrolytically cleave nucleic acids under mild conditions is currently attracting great interest in the field of artificial nucleases. While many reagents have been successfully applied to RNA hydrolytic cleavage, there have been fewer successes with DNA because of its relatively high hydrolytic stability. Metal ions play important roles in many enzyme and all ribozymemediated examples of these scission reactions. Small metal complexes that promote the hydrolytic cleavage of DNA, therefore could be useful not only in molecular biology and drug design but also in elucidating the precise role of metal ions in enzyme catalysis.In this dissertation, new types of macrocyclic polyamines containing nucleobases or polyamides and their metal complexes have been prepared. The synthetic methods and influencing factors about title compounds have been developed.The supramolecular recognition of macrocyclic polyamine metal complexes for bases by UV-visible spectrophotometric titration, ~1H NMR titration, and by ESI-MS.The results show that the host compounds form 1:2 supramolecular complexes with the guests. The experimental results indicate that the molecular recognition is mainly due to electrostatic force, π-π stacking and hydrogen bond.