Synthesis Of Rhodamine B-Cyclen Conjugates And DNA Cleavage By Their Metal Complexes

Artificial nuclease acid enzyme is named imitation enzyme compounds, it has the efficient catalysize like enzymes, and it is more simple and stable than enzyme. The people who study the oxidation and hydrolysis fracture mechanism of artificial nuclease acid enzyme on DNA for exploring the genetic information that is stored in the DNA, in order to explore the secret of life to establish theoretical basis for all sorts of genetic diseases, such as heal tumor. Therefore the research artificial nuclease enzymae has important theoretical significance and application value.This paper has designed and synthesized nine RhB derivatives, including three RhB-cyclen conjugates as target product. All the target compounds were confirmed through'H NMR,13C NMR and IR and ESI-MS.Through the agarose gel electrophoresis, preliminary studies on the cleavage of pUC19 DNA in the presence of Cu2+, Zn2+, Co2+complexes have been performed. We have investigated application of the target product in ion recognition discoloration fluorescence probe by the absorption spectrometry and fluorescence spectrum. Research results show that these Co2+ complexes have good cutting action on the pUC19 plasmid DNA. Meanwhile, through research and analysis we have investigated the target product have strong identification function to some ions. The ligands of 1C and 2C have strong fluorescence response to Cr3+ ion in acetonitrile, and the ligand 3C have strong fluorescence response to Pb2+. Furthermore, these fingdings lay a certain foundation for our team's other students in studying metal cations fluorescence probe by RhB derivatives ligands.