| Resolution of multicomponent overlapping signals is one of the challenging tasks in analytical chemistry studies. Both immune algorithm and wavelet transform are effective tools for resolution of multicomponent overlapping signals. In this dissertation, at first, the theory and algorithm of immune algorithm and wavelet transform are introduced and the applications of them in analytical chemistry are reviewed. Then, with the aim to develop new tools for resolution of overlapping analytical signals, the following works are carried out:Based on the wavelet compression and immune algorithm, a fast algorithm for resolution of 2-D multicomponent overlapping chromatogram is proposed. By application of the method in resolution and quantitative determination of multicomponent 2-D overlapping chromatograms, it is demonstrated that this method is fast in calculation speed and accurate in quantitative calculation. Therefore, the proposed algorithm may be an alternative effective method for resolution of multicomponent 2-D overlapping chromatogram.Based on the immune algorithm, an adaptive immune algorithm is proposed. With the establishment of the database of the standards peaks, the standard information is adaptively generated from the database for the resolution of the mixture sample. With the adoption of the correlation coefficient between mixture signal and standard signals and the optimal strategy, the algorithm is much more intelligentized and the efficiency of the algorithm is improved obviously. From the resolution results of a four-component NMR spectrum and a nine-component NMR spectrum, it is demonstrated that the proposed algorithm is an effective adaptive immune algorithm. Compare with the IA-GA, the calculation speed of using the AIA is much faster and the resolved result is much more accurate.With the aim of quantitative determination of multicomponent overlapping chromatograms, an improvement of the transmutation method was proposed with WT smoothing, cubic spline interpolation, and a new criterion for determination of the optimal points number. With the proposed algorithm, the oscillation generated in the transmutation process can be effectively suppressed. By the application to simulated... |
