Exploration And Research On Spectral Characteristic Of Human Blood

Spectroscopy technology applied to research the characteristics of biological tissue is one of the hot subjects in the fields of biomedicine and photons. The structures of biomacromolecules are researched by the characteristics of spectrums excitated by ray. The purpose is to illustrate the structures of biomacromolecules in solution by the endogenetic or exogenous fluorescence analysis. And this could be used to diagnose the pathological changes of biological tissue. The spectral characteristics of typical human blood samples are studied detailedly in this paper and the main research contents and innovations are as follows:1. The influences of temperature, concentration and wavelength on fluorescence spectra of serums are investigated systematically. The change law of fluorescence intensity of the serums along with the temperature is discussed by experiments. The fluorescence intensity of the serums changes with the concentration because of excitation fluorescence, concentration quenching and absorption.The line type and peak position of the fluorescence spectrum of the serums are irrelevant to the excitation wavelength and the fluorescence intensity is closely related to the excitation wavelength. The spectrum has two relatively strong emitter regions, and the central wavelengths are 330nm and 452nm separately. The research result has important reference value to the selection of serum temperature, serum concentration and excitation wavelength in the diagnosis technology for induced fluorescence spectrum.The microcosmic model for ultra weak chemiluminescence of serum is theoretically analyzed. The change laws of the luminescence intensity along with the blood sugar and the three acids glyceride are investigated in detail. The differences between the absorption spectra of abnormal serum and that of normal serum are analyzed carefully. The normal or abnormal concentration of three acids glyceride in serum can be estimated by the absorbency of the serum.2. The synchronous fluorescence spectrums of serums respectively with different concentration and different biochemical parameters are researched. To the serums with same wavelength difference and different biochemical parameters, the synchronous fluorescence spectrums of these serums present differences from that of normal serums. To the serums with different wavelength difference, the figures and the fine structures of the synchronous fluorescence spectrums show obvious differences. By using these differences, the abnormal serums can be distinguished from the normal serums. Therefore, it provides reference for clinical diagnosis. 3. The scattering spectrums of normal serums and abnormal serums are researched, and the scattering spectrums of serums with same biochemical parameters and different concentrations are also studied. The scattering spectrums of abnormal serums, such as the serums with hyperlipidemia and the serums with hyperglycemia, have obvious differences from the spectrums of normal serums on the characteristic peaks. The peak values augment along with the increase of the blood lipids concentration and the blood sugar concentration. Moreover, the relationship between I446/I464 and blood sugar concentration, and the relationship between I615/I628 and blood sugar concentration are nearly linear. However, there is no similar phenomenon in the scattering spectrums of serums with hyperlipidemia. Hence, it can be used to distinguish between the serums with hyperglycemia and the serums with hyperlipidemia.4. The fluorescence polarization spectrums of normal serums and abnormal serums are also studied in this paper. When the concentrations of the blood sugar and the blood lipids in the serums are changed, the degree of polarization in the spectrums will vary. In different fluorescence areas, the energy transformation mechanism between fluorophores and the variety of polarization degrees are analyzed detailed.5. The fractal theory used to calculate the fractal dimensions of the spectrums is put forward. The differential modes of the fractal dimensions of spectrums of different serums are compared to distinguish. It provides some references for the fractal theory being applied in spectral recognition.The research results of this paper are in favor of studying the interaction mechanism between light and blood. It provides experimental and academic references for disease diagnosis and cure by dint of the blood endogenetic or exogenous radiation characteristics, and it is also helpful to research the structure and the changing rule of biomacromolecules in blood.