Evaluation Of AFLP Marker And Molecular Biology Methods On Resource Plant

Resource plants bear countless valuable genes and to explore and utilize them are the important issues of botany research. Based on development demands of China Leymus chinensis (Trin.) Tzvel and Heliathus annuus L.were chosen for evaluation and analysis using molecular marker and molecular biological methods for future plant breeding.Chinese Leymus (Leymus chinensis (Trin.) Tzvel.), a member of Poaceae, is a dominate grass species distributed in the eastern grassland in Eurasia. It has an important role in stock raising and environmental protection in northern China. In recent years, due to poor scientific management, overgrazing and the"three-low-problem"(low seed setting, germination and tasseling), biodiversity of L. chinensis has degenerated which restricts the artificial grassland construction, natural grassland improvement and control of deserted land. Therefore, establishing core collection of Leymus chinensis, improving Chinese Leymus, fast evaluating and producing the new germplasm by biotechnology based on morphological investigation and molecular biology become the main subjects to be urgently solved. In this paper, initial works have been done from four aspects and obtained the results as listed as below:1. By morphological investigation and AFLP marker,the results showed that Leymus chinensis varies greatly morphologically but little genetically. Moreover, there is sharp difference between two ecotypes (grey-green and yellow-green) and the former has bigger variance than the latter has. Genetic variance of Leymus chinensis is related to long-term cultivation and geographic distribution.2. PCO and Path Analysis simplified the complicacy of 31 morphological traits analysis in Leymus chinensis. The result suggested that asexual reproductive growth depended on vegetative growth greatly.3. AFLP has a distinct advantage in biodiversity analysis, especially in analyzing not high polymorphism of Leymus chinensis. Further, cluster analysis together with PCA can evaluates the relationship of close genetic resources as well as further genetic resources.4. During the selection of primers for AFLP analysis in this study, few detectable bands were found when the EcoRI selected primers with selective bases TN at...