Identification And Characterization Of A Novel Mouse Erythroid Differentiation-Denucleation Related Gene (MEDDF)

Cell commitment and differentiation depend on the establishment of specific patterns of gene expression achieved through networks of regulatory factors. Cell-specific gene expression appears to reflect the interplay of multiple ubiquitous factors with a more limited array of tissue-restricted factors. The main features of mammalian erythroblast terminal differentiation are the expression of hemoglobin, pycnosis of nuclear chromatin and enucleation, thus give rise to anucleated reticulocytes. Enucleation is a kind of autonomous activity and a unique differentiation model, it occurs only in mammalian erythroblast but not in non-mammalian animals. The phylogenetic derivation and the molecular mechanism of these denucleation features have long been the fascinating unanswered questions in the field of erythropoiesis. It was known that a series of erythroid-specific hematopoietic factors, such as EPO, BSF-E, CSF-E, IL-3 and IL-6 can act on different stages of hematopoietic cells and mediate the programmed expression of the...