Rapid Enrichment And Selection Of Human Genomic Regulatory Sequences

As the sequencing of human genome goes on smoothly, it is imminent to investigate the function of genome. The main function of genome includes (a) the function of gene's transcripts, (b) the regulation of gene expression, (c) the organization of genome and (d) the evolution of genome. Coordinated gene expression and highly ordered structure of genome results from the interaction between DNA and protein. So it is important to develop new strategy and to establish efficient techniques to isolate and to identify the genomic regulatory sequences, which is the basis for the identification of DNA binding protein and for the study of the regulation of gene expression.Presently there lack efficient methods to broadly isolate and identify DNA regulatory fragments. Only a few of transcription factors were cloned because they are expressed in low level and it's difficult to obtain pure protein enough for amino acid sequencing or preparation of antibody. Their expression pattern and the interaction between transcription factor and DNA were not studied in detail. It's difficult to obtain valuable DNA regulatory information by computer software analysis. Thus little is known about the transcriptional regulation of most genes. A main task to study the transcriptional regulation is still to isolate and identify the regulatory DNA fragments and the corresponding transcription factors, to study the interaction between DNA and proteins.Several methods to screen and to identify DNA regulatory fragments have been developed. They are: (a) expression assay with continuously truncted DNA constructs, which is widely used to analyze the position and the activity of regulatory fragments, but it needs suitable restriction enzyme sites for DNA deletion. (b) analysis of the sensitivity...