Isolation Of Erythroid Differentiation Related CDNA

Erythropoiesis is a complicated process , which is controlled by the combined effects of growth factors and transcription factors. Hematopoietic growth factors, especially erythropoietin(EPO), play important roles in promotion of the survival, proliferation and differentiation of erythroid progenitor cells, and in the activation of expression of specific genes in the cells. Recently, many investigations have demonstrated that transcription factors are more crucial in the process of erythroid development. During the terminal differentiation of mammalian erythroblasts remarkable changes occur in both morphology and chemistry .The erythroid cells pass through a series of stages including of cease of cell division, decreasing of cell volume, nuclear chromatin condensation, expressing hemoglobin, enucleation. As the changes reflect some genes expressed herein, identification and characterization of the differentially expressed genes will be helpful to understanding the molecular mechanism of erythroid terminal differentiation.A model of murine erythroblasts infected with anemia-inducing Friend leukemia virus(FVA cells) has been developed using EPO-induced differentiation. In this model FVA erythroblasts undergo terminal differentiation relatively homogenously in vitro when EPO is added to the medium. Subtractive hybridization is a method to be used to isolate sequences presenting in one mRNA population but not in others.