Study On Cold Resistance Physiological Fcology Of Primula SieboldiiE.Morren

Physiological characters of northeast wild earlier spring flower named Primula Sieboldii E. Morren were researched under low temperature stress. Systemic study on Primula Sieboldii E. Morren were carried on membrane leakage and membrane-lipid peroxidation, contents of soluble protein and dissociative proline, changes of H202 and defensive enzyme, ultrastructure of mesophyll cell, photosynthesis and transpiration, The main purpose is to disclose the physiological ecology acclimation mechanisms. (1) The contents of MDA and membrane leakage increased while the temperature decreased. The contents of MDA and membrane leakage were higher in low temperature stress with sunshine than that with dark. Low temperature stress made MDA and membrane leakage increased, but the two guide line sensitivity became lower during low temperature stress procession. Low temperature stress increase cold resistance ability of Primula Sieboldii E. Morren. (2) The contents of soluble protein increased in relatively high above zero low temperature stress( over 10 ~C), while contents of soluble protein decreased in relatively low temperature stress(0 慍). Contents of soluble protein increased in low temperature pretreatment, contents of soluble protein relatively kept stabilization though soluble protein of pre-treated Primula Sieboldii E. Morren decreased during low temperature stress procession. Soluble protein became lower in low temperature stress with sunshine than that with dark. Content of dissociative proline increased significantly in low temperature stress, Content of dissociative proline also increased with the high intensively low temperature stress and prolonged low temperature stress time. The increase of dissociative proline and soluble protein had actively meanings on cold resistance promoting of Prim ula Sieboldii E. Morren. (3) Contents of H2 02 increased with the high intensively low temperature stress, it has corresponding and relativity to contents increasing of MDA and membrane leakage. Contents of H2 02 were higher in low temperature stress with sunshine than that with dark. (4) Activity of SOD and ASP increased with the high intensively low temperature stress, Activity of POD increased in relative high low temperature stress( 10 0C), while activity of POD decreased in low temperature stress(0~C). SOD and ASP increased its ability to clear away H2 02 to protect plant against injury in low temperature environment, While POD didn抰 change like that. After low temperature pretreatment, Activity of SOD, ASP and POD increased so that the above tree enzyme enhance their ability to adapt to environment. SOD and POD reduced its sensitivity to low temperature to keep plant away low temperature injury, ASP increased the stability of enzyme to protect plant against injury in low temperature. (5) With the temperature decreasing, some change in the ultrastructure of cells was be observed, the number of mitochondria increased, chloroplast expanded became circle shape. Lamellae structure of chloroplast became foul-up slightly in low temperature with sunshine. Plasma membrane infolding occurred after pretreatment and low temperature stress, one new structure looked like vacuole was recognized between plasma membrane and cytoderm, the emergence of that phenomena maybe concern with plasma membrane infolding and vacuole. We I think plasma membrane infolding was the phenomena of cold resistant plant, the emergence of structure looked like vacuole was the key to...