Experimental Study On The Effect Of Taohong Siwu Decoction On Autophagy-related Proteins In Mice With Skin Photoagin

Purpose:This experiment by establishing the photoaging mouse model,the intervention,observe the skin pathological morphological changes in the groups of mice,the distribution and number of autophagy markers LC3-Ⅱ,Beclin1,P62 related protein in the tissue,explore the influence of autophagy protein on the photoaging mice,laying the foundation for further clinical research,prevention and treatment of skin photoaging.Material and method:Forty SPF 8-week-old Kunming mice divided into four groups randomly,including blank control group,photoaging model group,Taohong Siwu soup group,and VE control group,10 in each group.After a week of conventional feeding,the back hair of the mice was removed,and the UVA and UVB UV lamp were radiated except the blank group.To establish a photoaging mouse model we should irradiate frequently,7 times a week for 2 hours.After successful modeling,the model group received normal saline,Taohong Siwu soup and peach group,and the VE group received VE solution by oral intervention once a day,with a dose of 0.4ml / only.The drug was administered for 4 weeks.After the last dose,kill the mice the next day and skin tissue from the back was used for each test.Skin morphology was observed by HE staining;LC3Ⅱ,Beclin-Ⅰ levels in mouse skin tissues by immunohistochemistry;expression levels of autophagy-related proteins LC3 Ⅱ,Beclin-Ⅰ,P62 by Western-blot.Results:1.Results of HE staining in miceCompared with the blank control group,the mice in the model group showed increased epidermal thickness,stratification and unclear boundaries,and the dermis fiber organization was disordered and uneven distribution,and inflammatory infiltration was seen.Compared with the model group,the mice had a relatively complete skin structure,reduced epidermis thickness,reduced inflammatory infiltration,and reduced fracture of collagen fiber bundles.In the VE group,the dermis tissue was arranged more orderly,with reduced epidermal thickness and decreased inflammatory infiltrate.2.The western-blot resultsCompared with the blank group,LC3 and Beclin-1 and P62 were increased(P < 0.01).VE group increased LC3,Beclin-1,P62 decreased(P < 0.01),Compared with VE group,there is no significant difference was found between the two groups.3.Immunohistochemistry resultsLC3-Ⅱ,Beclin1 decreased(P <0.01)(P <0.01);and the LC3-Ⅱ in the VE group(P<0.01);Compared with VE group,there is no significant difference was found between the two groups.Conclusion:1.UV irradiation can inhibit cellular autophagic activity.2.Taohong Siwu soup can enhance autophagy,thus inhibiting the aging process of UV-induced skin damage,but the specific mechanism needs to be further explored.